Muthuirulandi Sethuvel Dhiviya Prabaa, Veeraraghavan Balaji, Vasudevan Karthick, Devanga Ragupathi Naveen Kumar, Murugan Dhivya, Walia Kamini, Anandan Shalini
1Department of Clinical Microbiology, Christian Medical College, Vellore, Tamil Nadu 632004 India.
2Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research, New Delhi, 110 029 India.
Gut Pathog. 2019 Nov 6;11:55. doi: 10.1186/s13099-019-0334-5. eCollection 2019.
is ranked as the second leading cause of diarrheal disease worldwide. Though infection occurs in people of all ages, most of the disease burden constitutes among the children less than 5 years in low and middle income countries. Recent increasing incidence of drug resistant strains make this as a priority pathogen under the antimicrobial resistance surveillance by WHO. Despite this, only limited genomic studies on drug resistant exists. Here we report the first complete genome of clinical serotype 2a and strains using a hybrid approach of both long-read MinION (Oxford Nanopore Technologies) and short-read Ion Torrent 400 bp sequencing platforms. The utilization of this novel approach in the present study helped to identify the complete plasmid sequence of pSS1653 with structural genetic information of AMR genes such as II, A, R, - and - Identification of AMR genes in mobile elements in this human-restricted enteric pathogen is a potential threat for dissemination to other gut pathogens. The information on at genome level could help us to understand the genome dynamics of existing and emerging resistant clones.
它被列为全球腹泻病的第二大主要病因。尽管各年龄段的人都可能感染，但在低收入和中等收入国家，大部分疾病负担集中在5岁以下儿童中。最近耐药菌株发病率的上升使其成为世界卫生组织抗菌药物耐药性监测的重点病原体。尽管如此，关于耐药性的基因组研究仍然有限。在此，我们报告了使用长读长MinION（牛津纳米孔技术公司）和短读长Ion Torrent 400 bp测序平台的混合方法获得的临床血清型2a和菌株的首个完整基因组。本研究中这种新方法的应用有助于鉴定携带II、A、R、-和-等AMR基因结构遗传信息的pSS1653完整质粒序列。在这种人类限制性肠道病原体的移动元件中鉴定AMR基因对传播到其他肠道病原体是一个潜在威胁。基因组水平上的信息可以帮助我们了解现有和新出现的耐药克隆的基因组动态。
