Preidel A, Schimmer O
Institut für Botanik und Pharmazeutische Biologie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Curr Genet. 1986;10(5):393-5. doi: 10.1007/BF00418412.
We developed a test system with mutant strains of Chlamydomonas reinhardtii requiring arginine. Here, the meiotic recombination between two arginine loci served as an indicator of genotoxicity. After crossing, zygotes were treated with the alkylating agent dimethyl-nitrosamine (DMN) for 30 min. A dose dependent increase of wild type recombinants was found for DMN concentrations ranging from 68 microM to 680 microM. Like in cells of some higher plants, the agent was active in Chlamydomonas zygotes without any exogenous metabolic activation.
我们开发了一种针对需要精氨酸的莱茵衣藻突变株的测试系统。在此，两个精氨酸位点之间的减数分裂重组作为遗传毒性的指标。杂交后，合子用烷基化剂二甲基亚硝胺（DMN）处理30分钟。在68微摩尔至680微摩尔的DMN浓度范围内，发现野生型重组体呈剂量依赖性增加。与一些高等植物的细胞一样，该试剂在莱茵衣藻合子中具有活性，无需任何外源性代谢激活。
