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人肝癌细胞系中全氟辛酸毒性的测定

Determination of Perflourooctanoic Acid Toxicity in a Human Hepatocarcinoma Cell Line.

作者信息

Abudayyak Mahmoud, Öztaş Ezgi, Özhan Gül

机构信息

Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Turkey.

Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey.

出版信息

J Health Pollut. 2021 Aug 17;11(31):210909. doi: 10.5696/2156-9614-11.31.210909. eCollection 2021 Sep.

DOI:10.5696/2156-9614-11.31.210909
PMID:34434601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8383792/
Abstract

BACKGROUND

Perfluorooctanoic acid (PFOA) is used in different industrial and commercial products. Research shows the presence of PFOA in home dusts, tap and surface water, and in biological samples. The International Agency for Research on Cancer (IARC) has classified PFOA as a possible carcinogen for humans. The liver is thought to be a target organ of PFOA accumulation and toxicity.

OBJECTIVE

Some studies have found toxic effects on the liver and related mechanisms; however, more studies are needed to better understand PFOA - induced hepatotoxicity.

METHODS

In the present study, a human hepatocarcinoma cell line was exposed to PFOA for 24 hours and cell viability, apoptosis, the oxidative system and immune response were evaluated.

RESULTS

While apoptosis was the main cell death pathway at low concentration (86.5%), the necrotic cell fraction increased with higher concentrations (46.7%). Significant changes in the reactive oxygen species (5.3-folds) glutathione (GSH) (1.7-folds) and catalase (CAT) (1.4-folds) levels were observed, as well as changes to interleukin-6 (≤1.8-fold) and interleukin-8 levels (35-40%).

CONCLUSIONS

In light of the data, PFOA is potentially hepatotoxic through the investigated pathways. The results represent a background for future in vivo mechanistic studies.

COMPETING INTERESTS

The authors declare no competing financial interests.

摘要

背景

全氟辛酸（PFOA）用于不同的工业和商业产品中。研究表明，家庭灰尘、自来水和地表水以及生物样本中均存在PFOA。国际癌症研究机构（IARC）已将PFOA归类为对人类可能的致癌物。肝脏被认为是PFOA蓄积和毒性的靶器官。

目的

一些研究已发现PFOA对肝脏的毒性作用及其相关机制；然而，需要更多研究以更好地了解PFOA诱导的肝毒性。

方法

在本研究中，将一种人肝癌细胞系暴露于PFOA 24小时，并评估细胞活力、凋亡、氧化系统和免疫反应。

结果

低浓度时凋亡是主要的细胞死亡途径（86.5%），而坏死细胞比例随浓度升高而增加（46.7%）。观察到活性氧（5.3倍）、谷胱甘肽（GSH）（1.7倍）和过氧化氢酶（CAT）（1.4倍）水平有显著变化，白细胞介素-6（≤1.8倍）和白细胞介素-8水平也有变化（35 - 40%）。

结论

根据这些数据，PFOA可能通过所研究的途径具有肝毒性。这些结果为未来的体内机制研究提供了背景资料。

利益冲突

作者声明不存在相互竞争的财务利益。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/83ab39ebccc7/i2156-9614-11-31-210909-f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/ee99582b4f25/i2156-9614-11-31-210909-f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/2752002ed4d1/i2156-9614-11-31-210909-f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/0b6585e34a4f/i2156-9614-11-31-210909-f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/265c7e88155f/i2156-9614-11-31-210909-f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/74ec2df90cdf/i2156-9614-11-31-210909-f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/384a0b595dce/i2156-9614-11-31-210909-f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/83ab39ebccc7/i2156-9614-11-31-210909-f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/ee99582b4f25/i2156-9614-11-31-210909-f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/2752002ed4d1/i2156-9614-11-31-210909-f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/0b6585e34a4f/i2156-9614-11-31-210909-f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/265c7e88155f/i2156-9614-11-31-210909-f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/74ec2df90cdf/i2156-9614-11-31-210909-f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/384a0b595dce/i2156-9614-11-31-210909-f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae0c/8383792/83ab39ebccc7/i2156-9614-11-31-210909-f07.jpg

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Can sustained exposure to PFAS trigger a genotoxic response? A comprehensive genotoxicity assessment in mice after subacute oral administration of PFOA and PFBA.PFAS 能否引发持续暴露的遗传毒性反应？在亚急性经口给予 PFOA 和 PFBA 后对小鼠进行的综合遗传毒性评估。
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