Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.
Institute for Future Beverages, Research & Development Division, Kirin Holdings Company, Limited. 1-17-1, Namamugi, Tsurumi-ku, Yokohama, Kanagawa 230-8628, Japan.
Biosensors (Basel). 2021 Aug 12;11(8):272. doi: 10.3390/bios11080272.
Traceability analysis, such as identification and discrimination of yeasts used for fermentation, is important for ensuring manufacturing efficiency and product safety during brewing. However, conventional methods based on morphological and physiological properties have disadvantages such as time consumption and low sensitivity. In this study, the resistive pulse method (RPM) was employed to discriminate between and and and by measuring the ionic current response of cells flowing through a microsized pore. The height and shape of the pulse signal were used for the simultaneous measurement of the size, shape, and surface charge of individual cells. Accurate discrimination of from spp. was observed with a recall rate of 96.3 ± 0.8%. Furthermore, budding was quantitatively detected by evaluating the shape of the waveform of the current ionic blockade. We showed a proof-of-concept demonstration of RPM for the detection of contamination of spp. in and for monitoring the fermentation of through the quantitative detection of budding cells.
溯源分析，例如鉴定和区分用于发酵的酵母，对于确保酿造过程中的生产效率和产品安全性非常重要。然而，基于形态和生理特性的传统方法存在耗时和灵敏度低等缺点。在本研究中，我们采用电阻脉冲法（RPM）通过测量流经微孔的细胞的离子电流响应来区分 和 、 和 。脉冲信号的高度和形状用于同时测量单个细胞的大小、形状和表面电荷。观察到对 spp. 的准确区分，召回率为 96.3±0.8%。此外，通过评估电流离子阻断的波形形状来定量检测出芽 。我们通过定量检测出芽细胞来展示 RPM 用于检测 和 中 spp. 污染以及监测 通过发酵的概念验证演示。
