用于在食品样品中进行稳健先导检测的比率型 G-四链体分析。

Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples.

机构信息

College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China.

Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

出版信息

Biosensors (Basel). 2021 Aug 16;11(8):274. doi: 10.3390/bios11080274.

Lead (Pb) pollution is a serious food safety issue, rapid detection of Pb residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb supervision in food samples. Pb specific aptamer can bolster a transition of G-quadruplex structural response to Pb; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb testing associated with food safety.

铅（Pb）污染是一个严重的食品安全问题，快速检测食品中的 Pb 残留对于保证食品质量和安全至关重要。在这里，我们提出了比率型适体探针，允许在食品样品中进行强大的 Pb 监测。Pb 特异性适体能促进 G-四链体结构响应向 Pb 的转变；这个过程可以通过高度特异性 G-四链体的 N-甲基甲川叶绿素 IX（NMM）来监测。特别是，G-四链体特异性染料和末端标记荧光团的利用，使探针能够对 Pb 产生比率型信号输出，极大地提高了 Pb 检测的稳健性。比率型 G-四链体测定法允许使用单链 DNA 适体在室温下简便、一次性地检测 Pb。我们证明了它在检测新鲜鸡蛋和自来水中的 Pb 污染方面的可行性。比率型 G-四链体设计有望用于与食品安全相关的现场 Pb 测试。