Edinburgh Super Resolution Imaging Consortium (ESRIC), Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, United Kingdom.
MRC Harwell Institute, Mammalian Genetics Unit, Harwell Campus, Oxfordshire, United Kingdom.
Curr Protoc. 2021 Aug;1(8):e224. doi: 10.1002/cpz1.224.
Super-resolution (diffraction unlimited) microscopy was developed 15 years ago; the developers were awarded the Nobel Prize in Chemistry in recognition of their work in 2014. Super-resolution microscopy is increasingly being applied to diverse scientific fields, from single molecules to cell organelles, viruses, bacteria, plants, and animals, especially the mammalian model organism Mus musculus. In this review, we explain how super-resolution microscopy, along with fluorescence microscopy from which it grew, has aided the renaissance of the light microscope. We cover experiment planning and specimen preparation and explain structured illumination microscopy, super-resolution radial fluctuations, stimulated emission depletion microscopy, single-molecule localization microscopy, and super-resolution imaging by pixel reassignment. The final section of this review discusses the strengths and weaknesses of each super-resolution technique and how to choose the best approach for your research. © 2021 The Authors. Current Protocols published by Wiley Periodicals LLC.
超分辨率（衍射极限）显微镜技术在 15 年前被开发出来，其开发者在 2014 年被授予诺贝尔化学奖，以表彰他们的工作。超分辨率显微镜技术越来越多地应用于从单个分子到细胞器官、病毒、细菌、植物和动物等各种科学领域，特别是作为哺乳动物模型生物的小家鼠。在这篇综述中，我们将解释超分辨率显微镜技术以及从其发展而来的荧光显微镜技术如何帮助复兴光学显微镜。我们涵盖了实验计划和样本制备，并解释了结构光照明显微镜、超分辨率径向波动、受激发射损耗显微镜、单分子定位显微镜以及像素再分配的超分辨率成像。这篇综述的最后一部分讨论了每种超分辨率技术的优缺点，以及如何为您的研究选择最佳方法。© 2021 作者。本期 Wiley 出版社出版的《当代协议》。
