采用混合固相萃取-超高效液相色谱-串联质谱法快速测定港湾鼠海豚肝脏组织中的全氟和多氟烷基物质（PFAS）

Rapid Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Harbour Porpoise Liver Tissue by HybridSPE-UPLC-MS/MS.

作者信息

Trimmel Simone, Vike-Jonas Kristine, Gonzalez Susana V, Ciesielski Tomasz Maciej, Lindstrøm Ulf, Jenssen Bjørn Munro, Asimakopoulos Alexandros G

机构信息

Department of Chemistry, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.

Department of Biology, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway.

出版信息

Toxics. 2021 Aug 1;9(8):183. doi: 10.3390/toxics9080183.

A rapid hybrid solid phase extraction (HybridSPE) protocol tailored to ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) analysis was developed for the determination of 15 per- and polyfluoroalkyl substances (PFAS) in liver tissue from harbour porpoises (). The HybridSPE technique has been applied in trace concentration bioanalysis, but it was mainly used for liquid biological media until now. In this study, the protocol was applied on tissue matrix, and it demonstrated acceptable absolute recoveries (%) ranging from 44.4 to 89.4%. The chromatographic separation was carried out using a gradient elution program with a total run time of 4 min. The inter-day method precision ranged from 2.15 to 15.4%, and the method limits of detection (LODs) ranged from 0.003 to 0.30 ng/g wet weight (w.w.). A total of 20 liver samples were analyzed to demonstrate the applicability of the developed method in liver tissue from a wildlife species.

为测定港湾鼠海豚肝脏组织中的15种全氟和多氟烷基物质（PFAS），开发了一种适用于超高效液相色谱-电喷雾电离串联质谱（UPLC-ESI-MS/MS）分析的快速混合固相萃取（HybridSPE）方法。HybridSPE技术已应用于痕量浓度生物分析，但到目前为止主要用于液体生物介质。在本研究中，该方法应用于组织基质，其绝对回收率在44.4%至89.4%之间，结果令人满意。采用梯度洗脱程序进行色谱分离，总运行时间为4分钟。日内方法精密度在2.15%至15.4%之间，方法检测限（LOD）在0.003至0.30 ng/g湿重（w.w.）之间。共分析了20个肝脏样本，以证明所开发方法在野生动物肝脏组织中的适用性。