Wang Shanshan, Bai Miaomiao, Xu Kang, Shao Yirui, Yang Zhe, Xiong Xia, Huang Ruilin, Li Yao, Liu Hongnan
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Animals (Basel). 2021 Jul 27;11(8):2217. doi: 10.3390/ani11082217.
This study aimed to explore the effects of dietary coated cysteamine on oxidative stress and inflammation in diquat-induced weaning pigs. Twenty-four pigs were randomly assigned to three dietary groups with eight replicates: the control (fed base diet), diquat (fed base diet), and coated cysteamine + diquat groups (fed 80 mg/kg cysteamine). The experiment was conducted for 21 d, and consisted of a pre-starter period (14 d) and a starter period (7 d). Coated cysteamine treatment significantly increased ( < 0.05) the final weight and average daily gain (ADG) in pigs. The contents of alkaline phosphatase (ALP), immunoglobulin G (IgG), serine (Ser), and isoleucine (Ile) were elevated ( < 0.05) while the contents of albumin (ALB) and aspartic acid (Asp) were reduced ( < 0.05) in the serum after coated cysteamine supplementation. Coated cysteamine supplementation resulted in greater ( < 0.05) serum superoxide dismutase (SOD) activity, the expression of interleukin-10 (IL-10) mRNA in the colon, and the CuSOD mRNA expression in the jejunum ( < 0.05) and colon ( = 0.073). Coated cysteamine supplementation showed an increasing trend in villus height ( = 0.060), villus height/crypt depth (V/C) ( = 0.056), the expression levels of zonula occludens-1 (ZO-1) mRNA ( = 0.061), and Occludin mRNA ( = 0.074) in the jejunum. In summary, dietary supplementation with coated cysteamine improves the intestinal barrier function of the jejunum by increasing the immunoglobulin content and the relative expression of intestinal immune factor mRNA in pigs while alleviating oxidative stress and inflammatory reactions caused by diquat.
本研究旨在探讨日粮包被半胱胺对百草枯诱导的断奶仔猪氧化应激和炎症的影响。24头仔猪被随机分为三个日粮组，每组8个重复：对照组（饲喂基础日粮）、百草枯组（饲喂基础日粮）和包被半胱胺+百草枯组（饲喂80 mg/kg半胱胺）。试验持续21天，包括预启期（14天）和启期（7天）。包被半胱胺处理显著提高（<0.05）了仔猪的末重和平均日增重（ADG）。补充包被半胱胺后，血清中碱性磷酸酶（ALP）、免疫球蛋白G（IgG）、丝氨酸（Ser）和异亮氨酸（Ile）的含量升高（<0.05），而白蛋白（ALB）和天冬氨酸（Asp）的含量降低（<0.05）。补充包被半胱胺导致血清超氧化物歧化酶（SOD）活性增强（<0.05），结肠中白细胞介素-10（IL-10）mRNA的表达以及空肠（<0.05）和结肠（=0.073）中铜超氧化物歧化酶（CuSOD）mRNA的表达增加。补充包被半胱胺使空肠绒毛高度（=0.060）、绒毛高度/隐窝深度（V/C）（=0.056）、紧密连接蛋白-1（ZO-1）mRNA（=0.061）和闭合蛋白mRNA（=0.074）的表达水平呈增加趋势。总之，日粮中补充包被半胱胺可通过提高仔猪免疫球蛋白含量和肠道免疫因子mRNA的相对表达来改善空肠的肠道屏障功能，同时减轻百草枯引起的氧化应激和炎症反应。
