Wang Shanshan, Bai Miaomiao, Shu Qingyan, Liu Zhengan, Shao Yirui, Xu Kang, Xiong Xia, Liu Hongnan, Li Yao
Institute of Animal Nutrition, Northeast Agricultural University, Harbin, China.
Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Key Laboratory of Agro-ecological Processes in Subtropical Region; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.
Front Nutr. 2022 Apr 13;9:846684. doi: 10.3389/fnut.2022.846684. eCollection 2022.
Piglet enteritis is a major problem that needs to be solved urgently in modern pig production. Paeonol (Pae) has been used as a novel treatment option due to its good medicinal value. This study purported to elucidate the regulatory mechanism of Pae on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) in weaned piglets. A total of 36 crossbred (Duroc × Landrace × Yorkshire) weaned piglets were stochastically split into six groups: the control group, DSS group, 0.2% Pae group, 0.4% Pae group, 0.8% Pae group, and mesalazine group. The control and DSS groups were fed with a basic diet, the three Pae and mesalazine groups were fed with 0.2, 0.4, 0.8%, and 2 g mesalazine per kilogram of basic diet throughout the study. On the 15th day of the test period, the control group was gavaged with 10 ml of normal saline, while the remaining five groups were gavaged with 10 ml 5% DSS solution for 13 days. The study lasted for 27 days. The results showed that the 0.8% Pae group significantly increased the average daily feed intake (ADFI) and Occludin mRNA expression in the colon of piglets ( < 0.05). The 0.2% Pae group markedly increased the average daily gain (ADG) and zonula occludens-1 (ZO-1) mRNA expression ( < 0.05). In the 0.2% and 0.4% Pae groups, the feed-to-gain ratio (F/G) was significantly reduced and the mRNA expression levels of Caspase-8, respectively, markedly enhanced the mRNA expression levels of transforming growth factor-β (TGF-β) and interleukins-4 (IL-4) ( < 0.05). In the 0.8% Pae group, the relative abundance of was significantly reduced ( < 0.05). In the 0.4% Pae group, the relative abundance of was notably increased ( < 0.05). In the 0.2 and 0.8% Pae groups, the relative abundance of was markedly increased ( < 0.05). In the 0.2% Pae group, the contents of propionic acid, butyric acid, and valerate acid were markedly higher ( < 0.05). Thus, it is speculated that Pae may regulate the balance of anti-inflammatory/pro-inflammatory factors, improve intestinal tight junction expression, reduce apoptosis, and improve intestinal microflora structure and growth performance of piglets, thereby restoring intestinal barrier function and alleviating DSS-induced UC in piglets.
仔猪肠炎是现代养猪生产中亟待解决的主要问题。丹皮酚（Pae）因其良好的药用价值而被用作一种新型治疗选择。本研究旨在阐明Pae对葡聚糖硫酸钠（DSS）诱导的断奶仔猪溃疡性结肠炎（UC）的调控机制。将36头杂交（杜洛克×长白×约克夏）断奶仔猪随机分为六组：对照组、DSS组、0.2%Pae组、0.4%Pae组、0.8%Pae组和美沙拉嗪组。对照组和DSS组饲喂基础日粮，在整个研究过程中，三个Pae组和美沙拉嗪组每千克基础日粮分别添加0.2、0.4、0.8%的Pae和2g美沙拉嗪。在试验期第15天，对照组灌胃10ml生理盐水，其余五组灌胃10ml 5%DSS溶液，持续13天。研究持续27天。结果表明，0.8%Pae组显著提高了仔猪的平均日采食量（ADFI）和结肠中闭合蛋白mRNA表达（P<0.05）。0.2%Pae组显著提高了平均日增重（ADG）和紧密连接蛋白1（ZO-1）mRNA表达（P<0.05）。在0.2%和0.4%Pae组中，料重比（F/G）显著降低，Caspase-8的mRNA表达水平分别显著提高了转化生长因子-β（TGF-β）和白细胞介素-IL-4的mRNA表达水平（P<0.05）。在0.8%Pae组中，[此处原文缺失具体内容]的相对丰度显著降低（P<0.05）。在0.4%Pae组中，[此处原文缺失具体内容]的相对丰度显著增加（P<0.05）。在0.2%和0.8%Pae组中，[此处原文缺失具体内容]的相对丰度显著增加（P<0.05）。在0.2%Pae组中，丙酸、丁酸和戊酸的含量显著更高（P<0.05）。因此，推测Pae可能调节抗炎/促炎因子的平衡，改善肠道紧密连接表达，减少细胞凋亡，改善仔猪肠道微生物结构和生长性能，从而恢复肠道屏障功能，减轻DSS诱导的仔猪UC。
