与科特迪瓦共生和致病微生物存在相关的人类肠道微生物群组成分析。
Analysis of Human Gut Microbiota Composition Associated to the Presence of Commensal and Pathogen Microorganisms in Côte d'Ivoire.
作者信息
Di Cristanziano Veronica, Farowski Fedja, Berrilli Federica, Santoro Maristella, Di Cave David, Glé Christophe, Daeumer Martin, Thielen Alexander, Wirtz Maike, Kaiser Rolf, Eberhardt Kirsten Alexandra, Vehreschild Maria J G T, D'Alfonso Rossella
机构信息
Institute of Virology, Faculty of Medicine and University Hospital of Cologne, University of Cologne, 50935 Cologne, Germany.
Department I of Internal Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, 50937 Cologne, Germany.
出版信息
Microorganisms. 2021 Aug 18;9(8):1763. doi: 10.3390/microorganisms9081763.
BACKGROUND
The human gut microbiota is a microbial ecosystem contributing to the maintenance of host health with functions related to immune and metabolic aspects. Relations between microbiota and enteric pathogens in sub-Saharan Africa are scarcely investigated. The present study explored gut microbiota composition associated to the presence of common enteric pathogens and commensal microorganisms, e.g., and species, in children and adults from semi-urban and non-urban localities in Côte d'Ivoire.
METHODS
Seventy-six stool samples were analyzed for microbiota composition by 16S rRDNA sequencing. The presence of adeno-, entero-, parechoviruses, bacterial and protozoal pathogens, , and commensal species, was analyzed by different molecular assays.
RESULTS
Twelve individuals resulted negative for any tested microorganisms, 64 subjects were positive for one or more microorganisms. Adenovirus, enterovirus, enterotoxigenic (ETEC), and were frequently detected.
CONCLUSIONS
The bacterial composition driven by Prevotellaceae and Ruminococcaceae confirmed the biotype related to the traditional dietary and cooking practices in low-income countries. Clear separation in UniFrac distance in subjects co-harboring and was evidenced. Alpha diversity variation in negative control group versus only positive suggested its possible regulatory contribution on intestinal microbiota. Pathogenic bacteria and virus did not affect the positive outcome of co-harbored .
背景
人类肠道微生物群是一个微生物生态系统，通过与免疫和代谢方面相关的功能，有助于维持宿主健康。在撒哈拉以南非洲，微生物群与肠道病原体之间的关系鲜有研究。本研究探讨了与科特迪瓦半城市和非城市地区儿童及成人中常见肠道病原体和共生微生物（如 和 物种）的存在相关的肠道微生物群组成。
方法
通过16S rRDNA测序分析76份粪便样本的微生物群组成。通过不同的分子检测方法分析腺病毒、肠道病毒、副肠道病毒、细菌和原生动物病原体、 和共生 物种的存在情况。
结果
12名个体的所有检测微生物均呈阴性，64名受试者的一种或多种微生物呈阳性。腺病毒、肠道病毒、产肠毒素 （ETEC）和 经常被检测到。
结论
由普雷沃氏菌科和瘤胃球菌科驱动的细菌组成证实了与低收入国家传统饮食和烹饪习惯相关的生物型。在同时携带 和 的受试者中，在加权 UniFrac 距离上有明显分离。阴性对照组与仅 阳性组之间的α多样性差异表明其对肠道微生物群可能有调节作用。病原菌和病毒并未影响同时携带 的阳性结果。
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