Instituto de Investigación en Ciencias Biomédicas (IICB), CUCS, Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico.
Departamento de Reumatología, Hospital Civil de Guadalajara "Fray Antonio Alcalde", Guadalajara 44280, Jalisco, Mexico.
Molecules. 2021 Aug 17;26(16):4968. doi: 10.3390/molecules26164968.
Rheumatoid arthritis (RA) is an autoimmune inflammatory joint disease with complex pathogenesis associated with cytokine dysregulation. Macrophage migration inhibitory factor (MIF) plays a role in systemic inflammation and joint destruction in RA and could be associated with the secretion of other immune-modulatory cytokines such as IL-25, IL-31, and IL-33. For the above, our main aim was to evaluate the IL-25, IL-31, and IL-33 secretion from recombinant human MIF (rhMIF)-stimulated peripheral blood mononuclear cells (PBMC) of RA patients. The rhMIF and lipopolysaccharide (LPS) plus rhMIF stimuli promote the secretion of IL-25, IL-31, and IL-33 ( < 0.05) from PBMC of RA patients. The study groups, the different stimuli, and the interaction between both showed a statistically significant effect on the secretion of IL-25 ( < 0.05) and IL-31 ( < 0.01). The study of the effect of the RA patient treatments and their interaction with the effect of stimuli did not show an interaction between them. In conclusion, our study generates new evidence for the role of MIF in the secretion of IL-25, IL-31, and IL-33 and its immunomodulatory effect on RA.
类风湿关节炎（RA）是一种自身免疫性炎症性关节疾病，其发病机制复杂，与细胞因子失调有关。巨噬细胞移动抑制因子（MIF）在 RA 的全身炎症和关节破坏中起作用，并可能与其他免疫调节细胞因子如 IL-25、IL-31 和 IL-33 的分泌有关。基于此，我们的主要目的是评估重组人 MIF（rhMIF）刺激 RA 患者外周血单核细胞（PBMC）后 IL-25、IL-31 和 IL-33 的分泌情况。rhMIF 和脂多糖（LPS）加 rhMIF 刺激均可促进 RA 患者 PBMC 中 IL-25、IL-31 和 IL-33 的分泌（<0.05）。研究组、不同刺激物以及两者之间的相互作用对 IL-25（<0.05）和 IL-31（<0.01）的分泌均有统计学显著影响。研究 RA 患者的治疗及其与刺激物作用之间的相互作用，并未显示出两者之间的相互作用。总之，本研究为 MIF 在 IL-25、IL-31 和 IL-33 的分泌及其对 RA 的免疫调节作用提供了新的证据。
