Al-Odaini Najwa, Li Xiu-Ying, Li Bing-Kun, Chen Xing-Chun, Huang Chun-Yang, Lv Chun-Ying, Pan Kai-Su, Zheng Dong-Yan, Zheng Yan-Qing, Liao Wan-Qing, Cao Cun-Wei
Department of Dermatology and Venerology, First Affiliated Hospital, Guangxi Medical University, Nanning, China.
Guangxi Health Commission Key Lab of Fungi and Mycosis Research and Prevention, Nanning, China.
Front Microbiol. 2021 Aug 10;12:708280. doi: 10.3389/fmicb.2021.708280. eCollection 2021.
This study analyzed the drug sensitivity of spp. from Guangxi, Southern China. One hundred three strains of were recovered from 86 patients; 14 were HIV positive and 72 were HIV negative. Ninety-two strains were identified as var. , while 11 strains were identified as (5 and 6 ). The recovered strains were tested against commonly used antifungal drugs (fluconazole, amphotericin B, 5-fluorocytosine, itraconazole, and voriconazole) and to novel antifungal drugs (posaconazole and isavuconazole) using CLSI M27-A4 method. The results showed that all isolates were susceptible to most antifungal drugs, of which the minimum inhibitory concentration (MIC) ranges were as follows: 0.05-4 μg/ml for fluconazole, 0.25-1 μg/ml for amphotericin B; 0.0625-2 μg/ml for 5-fluorocytosine, 0.0625-0.25 μg/ml for itraconazole, 0.0078-0.25 μg/ml for voriconazole, 0.0313-0.5 μg/ml for posaconazole, 0.0020-0.125 μg/ml for isavuconazole for var. isolates, and 1-16 μg/ml for fluconazole, 0.125-1 μg/ml for 5-fluorocytosine, 0.25-1 μg/ml for amphotericin B, 0.0625-0.25 μg/ml for itraconazole, 0.0156-0.125 μg/ml for voriconazole, 0.0156-0.25 μg/ml for posaconazole, and 0.0078-0.125 μg/ml for isavuconazole for isolates. Furthermore, some var. isolates were found to be susceptible-dose dependent to 5-fluorocytosine and itraconazole. In addition, a reduction in the potency of fluconazole against is possible. We observed no statistical differences in susceptibility of var. and in the tested strains. Continuous observation of antifungal susceptibility of isolates is recommended to monitor the emergence of resistant strains.
本研究分析了中国南方广西地区某菌属的药敏情况。从86名患者中分离出103株该菌；其中14例为HIV阳性，72例为HIV阴性。92株被鉴定为某变种，11株被鉴定为另一菌种（5型和6型）。采用CLSI M27 - A4方法，将分离出的菌株针对常用抗真菌药物（氟康唑、两性霉素B、5 - 氟胞嘧啶、伊曲康唑和伏立康唑）以及新型抗真菌药物（泊沙康唑和艾沙康唑）进行测试。结果显示，所有分离株对大多数抗真菌药物敏感，其最低抑菌浓度（MIC）范围如下：氟康唑为0.05 - 4μg/ml，两性霉素B为0.25 - 1μg/ml；5 - 氟胞嘧啶为0.0625 - 2μg/ml，伊曲康唑为0.0625 - 0.25μg/ml，伏立康唑为0.0078 - 0.25μg/ml，泊沙康唑为0.0313 - 0.5μg/ml，艾沙康唑为0.0020 - 0.125μg/ml（针对某变种分离株）；氟康唑为1 - 16μg/ml，5 - 氟胞嘧啶为0.125 - 1μg/ml，两性霉素B为0.25 - 1μg/ml，伊曲康唑为0.0625 - 0.25μg/ml，伏立康唑为0.0156 - 0.125μg/ml，泊沙康唑为0.0156 - 0.25μg/ml，艾沙康唑为0.0078 - 0.125μg/ml（针对另一菌种分离株）。此外，发现一些某变种分离株对5 - 氟胞嘧啶和伊曲康唑呈剂量依赖性敏感。另外，氟康唑对该另一菌种的效力可能降低。我们观察到在测试菌株中某变种和另一菌种的药敏情况无统计学差异。建议持续观察该另一菌种分离株的抗真菌药敏情况以监测耐药菌株的出现。
