Bonek Krzysztof, Kuca Warnawin Ewa, Kornatka Anna, Plebańczyk Magdalena, Burakowski Tomasz, Maśliński Włodzimierz, Wisłowska Małgorzata, Głuszko Piotr, Ciechomska Marzena
Department of Rheumatology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland.
Department of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, 02-637 Warsaw, Poland.
Biomedicines. 2022 Apr 13;10(4):893. doi: 10.3390/biomedicines10040893.
This study aimed to investigate the associations of microRNA (miRs) signatures with cytokines, serum lipids, and disease activity in patients with psoriatic arthritis (PsA), ankylosing spondylitis (AS), and rheumatoid arthritis (RA). In total, 65 patients (PsA = 25, AS = 25, RA = 15) and 25 healthy controls (HC) were enrolled into the study. The expression of miR-223-5p, miR-92b-3p, miR-485-3p, miR-10b-5p, let-7d-5p, miR-26a-2-3p, miR-146b-3p, and cytokines levels were measured in sera. DIANA-mirPath analysis was used to predict pathways targeted by the dysregulated miRs. Disease activity scores were calculated. Lipid profile, uric acid, glucose level, and C-reactive protein (CRP) concentrations were determined in the blood. Based on lipid profiles, the PsA group had hypertriglyceridaemia, and RA patients revealed mixed dyslipidaemia, while in AS, no specific changes were found. miR expression analysis revealed upregulation of miR-26a-2-3p and miR-10b-5p in PsA, miR-485-3p in AS, and let-7d-5p in RA. Several correlations between disease activity indexes, metabolites levels, and expression of miRs were observed in PsA, RA, and AS patients. Finally, in ROC analysis, miR-26a-2-3p/miR-485-3p, and let-7d-5p/miR-146b-3p tandems revealed high sensitivity and specificity in distinguishing between PsA, AS, and RA. Our study illustrates the superiority of miR expressions in distinguishing between RA, PsA, and AS. In PsA, a unique regulatory pathway exists through miR-26a-2-3p, miR-223-5p, miR-10b-5p, and miR-92b-3p that converges proatherogenic metabolism and disease activity.
本研究旨在调查微小RNA（miRs）特征与银屑病关节炎（PsA）、强直性脊柱炎（AS）和类风湿关节炎（RA）患者的细胞因子、血脂及疾病活动度之间的关联。总共65例患者（PsA = 25例，AS = 25例，RA = 15例）和25名健康对照者（HC）纳入本研究。检测血清中miR-223-5p、miR-92b-3p、miR-485-3p、miR-10b-5p、let-7d-5p、miR-26a-2-3p、miR-146b-3p的表达及细胞因子水平。采用DIANA-mirPath分析预测失调miRs靶向的通路。计算疾病活动评分。测定血液中的血脂谱、尿酸、血糖水平及C反应蛋白（CRP）浓度。基于血脂谱，PsA组有高甘油三酯血症，RA患者有混合性血脂异常，而AS未发现特异性变化。miR表达分析显示，PsA中miR-26a-2-3p和miR-10b-5p上调，AS中miR-485-3p上调，RA中let-7d-5p上调。在PsA、RA和AS患者中观察到疾病活动指数、代谢物水平与miRs表达之间的若干相关性。最后，在ROC分析中，miR-26a-2-3p/miR-485-3p和let-7d-5p/miR-146b-3p组合在区分PsA、AS和RA方面显示出高敏感性和特异性。我们的研究说明了miR表达在区分RA、PsA和AS方面的优越性。在PsA中，通过miR-26a-2-3p、miR-223-5p、miR-10b-5p和miR-92b-3p存在一条独特的调控通路，该通路汇聚了促动脉粥样硬化代谢和疾病活动。
