Zhu Haifeng, Xiao Kun, Pu Wenjiang, Liu Zhenhua, Wang Yan, Gao Chaoyuan, Luo Sifang, Xu Yue, Guan Pingyin, Hu Jianfang
Laboratory of Fruit Physiology and Molecular Biology, China Agricultural University, Beijing 100193, China.
Plants (Basel). 2021 Jul 29;10(8):1561. doi: 10.3390/plants10081561.
The root-knot nematode () causes huge economic losses in the agricultural industry throughout the world. Control methods against these polyphagous plant endoparasites are sparse, the preferred one being the deployment of plant cultivars or rootstocks bearing resistance genes against species. Our previous study has cloned one resistance gene, , from Xinjiang wild myrobalan plum (). However, the function of remains elusive. In the present study, we have investigated the regulatory mechanism of in plant defense responses to . Our results indicate that fewer giant cells were detected in the roots of the transgenic tobacco than wild tobacco lines after incubation with . Transient transformations of full-length and TN structural domains of have induced significant hypersensitive responses (HR), suggesting that TIR domain might be the one which caused HR. Further, yeast two-hybrid results revealed that the full-length and LRR domain of could interact with the transcription factor . The addition of increased the ROS levels and induced HR. Thus, our data revealed that the LRR structural domain of may be associated with signal transduction. Moreover, we did not find any relative inductions of defense-related genes , and in , which has been incubated with . In summary, our work has shown the key functional domain of in the regulation of defense responses to in .
根结线虫在全球农业产业中造成巨大经济损失。针对这些多食性植物内寄生线虫的防治方法稀少，首选方法是部署携带抗根结线虫物种抗性基因的植物品种或砧木。我们之前的研究已从新疆野生樱桃李中克隆出一个抗性基因。然而，该基因的功能仍不清楚。在本研究中，我们研究了该基因在植物对根结线虫防御反应中的调控机制。我们的结果表明，用根结线虫孵育后，该转基因烟草根部检测到的巨型细胞比野生烟草品系少。该基因全长和TN结构域的瞬时转化诱导了显著的过敏反应（HR），表明TIR结构域可能是导致过敏反应的结构域。此外，酵母双杂交结果显示，该基因全长和LRR结构域可与转录因子相互作用。添加该基因增加了活性氧水平并诱导了过敏反应。因此，我们的数据表明该基因的LRR结构域可能与信号转导有关。此外，在用根结线虫孵育的该基因中，我们未发现防御相关基因、和的任何相关诱导。总之，我们的工作揭示了该基因在调控植物对根结线虫防御反应中的关键功能结构域。
