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野生茄子 Solanum aculeatissimum NBS-LRR 基因的克隆与鉴定及其与南方根结线虫抗性的关系

Molecular Cloning and Characterization of a Wild Eggplant Solanum aculeatissimum NBS-LRR Gene, Involved in Plant Resistance to Meloidogyne incognita.

机构信息

Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

出版信息

Int J Mol Sci. 2018 Feb 15;19(2):583. doi: 10.3390/ijms19020583.

DOI:10.3390/ijms19020583
PMID:29462897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5855805/
Abstract

Root-knot nematodes, spp., cause considerable damage in eggplant production. Transferring of resistance genes from wild relatives would be valuable for the continued improvement of eggplant. , a wild relative of eggplant possessing resistance to , is potentially useful for genetically enhancing eggplant. In the present study, we have isolated and characterized a nucleotide-binding site leucine-rich repeat (NBS-LRR) resistance gene, designated as . The full-length cDNA of the gene was obtained using the technique of rapid-amplification of cDNA ends (RACE). The open reading frame of the gene was 4014 bp and encoded a protein of 1338 amino acids. Sequence analysis indicated that belong to the non- Toll/Interleukin-1 receptor (TIR)-NBS-LRR type disease-resistance genes. Interestingly, quantitative RT-PCR showed that is expressed at low levels in uninfected roots, but was up-regulated by infection with . To investigate the role of in resistance against , the tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) system was used. Silencing of enhanced susceptibility of plants to , suggesting the possible involvement of in resistance against infection.

摘要

根结线虫， spp.，对茄子生产造成了相当大的损害。从野生亲缘种转移抗性基因对于茄子的持续改良将是有价值的。茄子的野生亲缘种，对，具有抗性，对于茄子的遗传增强具有潜在的用途。在本研究中，我们已经分离并鉴定了一个核苷酸结合位点富含亮氨酸重复（NBS-LRR）抗性基因，命名为。使用快速扩增 cDNA 末端（RACE）技术获得了该基因的全长 cDNA。基因的开放阅读框为 4014 bp，编码一个 1338 个氨基酸的蛋白质。序列分析表明，属于非 Toll/白细胞介素-1 受体（TIR）-NBS-LRR 型疾病抗性基因。有趣的是，定量 RT-PCR 显示，在未感染的根中表达水平较低，但被感染后上调。为了研究在对的抗性中的作用，我们使用了烟草脆裂病毒（TRV）介导的病毒诱导基因沉默（VIGS）系统。的沉默增强了对的敏感性，这表明可能参与了对的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/590d/5855805/1d5249e58080/ijms-19-00583-g006.jpg
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