Lim Hyeon-Ji, Jie Eun Yee, Park In-Sun, Kim Sang-Jun, Ahn Woo Seok, Jeong Seung-Il, Kim Suk Weon, Jung Chan-Hun
Jeonju AgroBio-Materials Institute, Jeonju-si 54810, Jeollabuk-do, Korea.
Biological Resource Center, Korea Research Institute of Bioscience & Biotechnology, Jeoneup-si 56212, Jeollabuk-do, Korea.
Plants (Basel). 2021 Aug 9;10(8):1635. doi: 10.3390/plants10081635.
is used in folk medicine to treat pain and allergic syndromes in Korea. However, the antibacterial and anti-inflammatory activities of callus extract remain unexplored. In this study, we aimed to evaluate the callus of pharmacological activity. Therefore, we first established in vitro calluses of via plant tissue culture methods. We then evaluated the antioxidant and anti-inflammatory effects of callus extract in lipopolysaccharide (LPS)-treated RAW264.7 macrophage cells. The callus extract showed antioxidant and anti-inflammatory effects. These effects were regulated via suppression of mitogen-activated protein kinase signaling through LPS-induced translocation of nuclear factor kappa B (NF-κB) p65 from the cytoplasm to the nucleus. callus extract also showed antibacterial and anti-inflammatory activities in -treated HaCaT keratinocyte cells. These results indicate that callus extract has antioxidant, antibacterial and anti-inflammatory activities, suggesting its possible application in the treatment of inflammatory disorders.
在韩国民间医学中用于治疗疼痛和过敏综合征。然而，愈伤组织提取物的抗菌和抗炎活性尚未得到探索。在本研究中，我们旨在评估愈伤组织的药理活性。因此，我们首先通过植物组织培养方法建立了体外愈伤组织。然后，我们评估了愈伤组织提取物在脂多糖（LPS）处理的RAW264.7巨噬细胞中的抗氧化和抗炎作用。愈伤组织提取物显示出抗氧化和抗炎作用。这些作用是通过抑制丝裂原活化蛋白激酶信号传导来调节的，该信号传导是通过LPS诱导核因子κB（NF-κB）p65从细胞质转移到细胞核来实现的。愈伤组织提取物在LPS处理的HaCaT角质形成细胞中也显示出抗菌和抗炎活性。这些结果表明，愈伤组织提取物具有抗氧化、抗菌和抗炎活性，表明其可能在炎症性疾病的治疗中得到应用。
