Fatur Karsten, Ravnikar Matjaž, Kreft Samo
Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, 32 Tržaška cesta, 1000 Ljubljana, Slovenia.
Plants (Basel). 2021 Aug 10;10(8):1643. doi: 10.3390/plants10081643.
The present research sought to compare the content of hyoscyamine/atropine and scopolamine in and its contested variety, , with the aim of investigating differences that may be of taxonomical significance. A multi-phase liquid extraction and high-performance liquid chromatography were used to extract and analyse these alkaloids in different organs from plants collected over two years at three sites. Our results showed that hyoscyamine was almost twice as prevalent as scopolamine across our 87 samples. The differences between organ types were large, but so too were intra-organ differences; differences due to organs proved to be significant for hyoscyamine, while they were only marginally significant for scopolamine. The collection site also proved to have a significant influence, but only on hyoscyamine content. The year of collection and the variety proved to not be significant. Our results support the theory that these two varieties are likely one, a view argued by many others, though more work is needed to draw concrete taxonomical conclusions.
本研究旨在比较天仙子及其有争议的变种中东莨菪碱/阿托品和东莨菪碱的含量，以调查可能具有分类学意义的差异。采用多相液体萃取和高效液相色谱法，对在三个地点两年内采集的植物不同器官中的这些生物碱进行萃取和分析。我们的结果表明，在我们的87个样本中，莨菪碱的含量几乎是东莨菪碱的两倍。器官类型之间的差异很大，但器官内部的差异也很大；事实证明，器官差异对莨菪碱来说是显著的，而对东莨菪碱来说只是略微显著。采集地点也被证明有显著影响，但仅对莨菪碱含量有影响。采集年份和变种被证明没有显著影响。我们的结果支持了这两个变种可能是同一个变种的理论，许多其他人也持有这一观点，不过还需要更多的研究才能得出具体的分类学结论。
