Fatur Karsten, Ravnikar Matjaž, Fras Vitjan, Kreft Samo
Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia.
Plants (Basel). 2022 Jan 4;11(1):126. doi: 10.3390/plants11010126.
The present article sought to evaluate the efficiency of various folk preparation methods commonly used in Europe for employing anticholinergic Solanaceae plants. The study aimed to uncover which folk methods were effective for the extraction of the anticholinergic tropane alkaloids of these plants, atropine and scopolamine. The folk extractions that were tested sought to simulate the preparation of teas, cold-water infusions, unguents, tinctures, fortified wines, and smoking. All preparation types and a control were then put through an extraction process to see what amount of the alkaloids had been maintained. These extractions were then analysed using high-performance liquid chromatography (HPLC). Cold- and hot-water preparations, tinctures, and fortified wines all proved to be effective means of extracting atropine and scopolamine from plant material under conditions seen in folk usage. Smoking and the oil-based unguent, however, yielded no alkaloids, suggesting a lack of efficiency for these preparations, a problem with our methodology, or possible chemical changes and losses associated with the preparation procedure.
本文旨在评估欧洲常用的各种民间制备方法在使用含抗胆碱能物质的茄科植物方面的效率。该研究旨在找出哪些民间方法能有效提取这些植物中的抗胆碱能托烷生物碱——阿托品和东莨菪碱。所测试的民间提取物试图模拟茶、冷水浸泡液、软膏、酊剂、强化葡萄酒和吸烟的制备过程。然后对所有制备类型和一个对照进行提取过程，以观察生物碱的保留量。接着使用高效液相色谱法（HPLC）对这些提取物进行分析。在民间使用的条件下，冷水和热水制剂、酊剂和强化葡萄酒都被证明是从植物材料中提取阿托品和东莨菪碱的有效方法。然而，吸烟和油基软膏未产生生物碱，这表明这些制备方法效率低下、我们的方法存在问题，或者与制备过程相关的可能化学变化和损失。
