Chen Xingyu, Li Yongyun, Yao Tengteng, Jia Renbing
Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, China.
Front Cell Dev Biol. 2021 Feb 11;9:616551. doi: 10.3389/fcell.2021.616551. eCollection 2021.
As a promising tool for cancer research, zebrafish have been widely applied in various tumor studies. The zebrafish xenograft model is a low-cost, high-throughput tool for cancer research that can be established quickly and requires only a small sample size, which makes it favorite among researchers. Zebrafish patient-derived xenograft (zPDX) models provide promising evidence for short-term clinical treatment. In this review, we discuss the characteristics and advantages of zebrafish, such as their transparent and translucent features, the use of vascular fluorescence imaging, the establishment of metastatic and intracranial orthotopic models, individual pharmacokinetics measurements, and tumor microenvironment. Furthermore, we introduce how these characteristics and advantages are applied other in tumor studies. Finally, we discuss the future direction of the use of zebrafish in tumor studies and provide new ideas for the application of it.
作为癌症研究中一种很有前景的工具，斑马鱼已被广泛应用于各种肿瘤研究。斑马鱼异种移植模型是一种低成本、高通量的癌症研究工具，能够快速建立且仅需少量样本，这使其受到研究人员的青睐。斑马鱼患者来源异种移植（zPDX）模型为短期临床治疗提供了有前景的证据。在本综述中，我们讨论了斑马鱼的特征和优势，如它们的透明和半透明特性、血管荧光成像的应用、转移性和颅内原位模型的建立、个体药代动力学测量以及肿瘤微环境。此外，我们介绍了这些特征和优势在其他肿瘤研究中的应用方式。最后，我们讨论了斑马鱼在肿瘤研究中的未来应用方向，并为其应用提供新的思路。
