Department of Bacteriology and Toxinology, Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany.
Viruses. 2021 Jul 27;13(8):1462. doi: 10.3390/v13081462.
Bacteriophage receptor binding proteins (RBPs) are employed by viruses to recognize specific surface structures on bacterial host cells. Recombinant RBPs have been utilized for detection of several pathogens, typically as fusions with reporter enzymes or fluorescent proteins. Identification of , the etiological agent of anthrax, can be difficult because of the bacterium's close relationship with other species of the group. Here, we facilitated the identification of using two implementations of enzyme-linked phage receptor binding protein assays (ELPRA). We developed a single-tube centrifugation assay simplifying the rapid analysis of suspect colonies. A second assay enables identification of suspect colonies from mixed overgrown solid (agar) media derived from the complex matrix soil. Thus, these tests identified vegetative cells of with little processing time and may support or confirm pathogen detection by molecular methods such as polymerase chain reaction.
噬菌体受体结合蛋白 (RBP) 被病毒用于识别细菌宿主细胞表面的特定结构。重组 RBP 已被用于检测多种病原体，通常与报告酶或荧光蛋白融合使用。由于炭疽杆菌与 属的其他物种密切相关，因此炭疽病病原体的鉴定可能较为困难。在这里，我们使用两种酶联噬菌体受体结合蛋白检测法 (ELPRA) 的实现来促进炭疽病的鉴定。我们开发了一种单管离心检测法，简化了对可疑菌落的快速分析。第二种检测法可以从源自复杂基质土壤的混合过度生长的固体 (琼脂) 培养基中鉴定出可疑的菌落。因此，这些测试用很少的处理时间就能鉴定出炭疽杆菌的营养细胞，并且可以支持或确认聚合酶链反应等分子方法的病原体检测。
