甘醇酸会加剧乳酸分析检测的性能表现。

Glycolic Acid Exacerbates the Analytical Performance of Lactate Assay.

机构信息

Department of Pathology, The MetroHealth System, Cleveland, OH, USA.

Department of Pathology, The MetroHealth System, Cleveland, OH, USA

出版信息

Ann Clin Lab Sci. 2021 Jul;51(4):584-586.

Abstract

In this study, we evaluated the analytical interference of glycolic acid on several lactate assays that use lactate oxidase and dehydrogenase. Herein, we tested the effect of different concentrations of glycolic acid (0.01-46mM) on the lactate assay by using central lab and point of care (POCT) analyzers: Radiometer ABL 800, Beckman AU480, Roche Cobas c502, and Abbott i-STAT. Glycolic acid concentrations as low as 0.12mM resulted in a ≥20% positive bias in lactate assay on the ABL 800 and a concentration of approximately 0.23mM resulted in >20% on the Roche Cobas c502 and Abbott i-STAT. A significant lactate gap is found at concentrations >0.06mM between the Radiometer ABL 800 and Roche Cobas c502/Abbott i-STAT. However, at concentrations ≥0.92mM, the lactate gap is very significant among all three platforms. Falsely elevated lactate levels could result in misdiagnosis.

摘要

在这项研究中，我们评估了几种使用乳酸氧化酶和脱氢酶的乳酸检测方法中，乙醇酸对分析的干扰。为此，我们使用中心实验室和即时检验（POCT）分析仪（Radiometer ABL 800、Beckman AU480、Roche Cobas c502 和 Abbott i-STAT）测试了不同浓度（0.01-46mM）乙醇酸对乳酸检测的影响。结果显示，ABL 800 上的乙醇酸浓度低至 0.12mM 时，乳酸检测的正偏差就≥20%，而罗氏 Cobas c502 和 Abbott i-STAT 上的乙醇酸浓度约为 0.23mM 时，乳酸检测的正偏差>20%。在浓度>0.06mM 时，ABL 800 和罗氏 Cobas c502/Abbott i-STAT 之间的乳酸差值显著。然而，在浓度≥0.92mM 时，三种平台之间的乳酸差值非常显著。错误升高的乳酸水平可能导致误诊。