Department of International Professional in Culinary Art, Faculty of International Hospitality Industry, Dusit Thani College, Bangkok, 10250, Thailand.
Food Technology and Innovation Research Centre of Excellence, Department of Food Industry, School of Agricultural Technology and Food Industry, Walailak University, Thasala, Nakhon Si Thammarat, 80161, Thailand.
Sci Rep. 2021 Aug 27;11(1):17320. doi: 10.1038/s41598-021-96694-w.
Impact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40-60%) and times (10-30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50-60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.
研究了超声辅助提取工艺（UAP）对 Bombay locusts（BL）（Patanga succinta L.）中蛋白质的产量、功能特性、抗氧化特性和分子特性的影响。在水提取过程中，对不同的超声辅助提取工艺条件（40-60%的不同振幅和 10-30 分钟的不同时间）进行了实验。值得注意的是，与典型工艺（TP）（在室温下以 100rpm 连续搅拌 1 小时）相比，UAP 可以提高产量和蛋白质回收率。UAP 条件的变化控制着 BL 的表面疏水性和游离α-氨基含量的变化。UAP 可以提高 BL 的溶解度，特别是在 pH 值高于 2 的水平下。UAP 对 BL 的泡沫能力和稳定性没有显著影响（p>0.05）。然而，UAP，特别是在 50-60%的振幅下，会影响 BL 的乳化活性和稳定性。UAP 为 BL 提供了高的自由基清除活性和良好的电子供体能力，特别是在 60%振幅下处理 20 分钟（UAP-60/20）时。与 TP 相比，UAP-60/20 对 BL 的等电点和分子特性的变化产生了影响，这可以通过傅里叶变换红外光谱（FTIR）进行监测。此外，BL 也是必需和非必需氨基酸的极好来源。因此，UAP 可以提高 BL 的提取效率，使 BL 具有良好的功能和抗氧化特性。
