Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang, 524025, China.
Mol Biol Rep. 2021 Oct;48(10):6897-6909. doi: 10.1007/s11033-021-06692-4. Epub 2021 Aug 28.
Environmental hypoxia affects the survival and development of organisms. It is also an important environmental factor that leads to oxidative damage. Hypoxia is a condition in which tissues are deprived of oxygen; reoxygenation is the phenomenon in which hypoxic tissues are exposed to oxygen. Hypoxia-reoxygenation is vital in pathogenesis, where the production of reactive oxygen species and antioxidant disparity significantly contribute to disease progression, and it is one of the most common physiological stressors in the aquaculture industry.
In this study, the full length of complementary DNA (cDNA) of the manganese superoxide dismutase (Mn-SOD) gene of healthy cobia Rachycentron canadum was analysed using rapid amplification of cDNA ends. The real-time quantitative Polymerase Chain Reaction was used to measure the expression levels of Mn-SOD mRNAs in various tissues (heart, muscle, brain, liver, kidney, gill, intestine, and spleen). The 2 method was used to performed the expression analysis. The experimental data were analysed using SPSS ver. 19.0 ( https://spss.software.informer.com/19.0/ ). P < 0.05 and P < 0.01 were set as significant differences. The values were articulated as mean ± standard deviation. The Mn-SOD gene cDNA sequence was 1209 bp long, including a 684 bp open reading frame, 42 bp 5'UTR and 483 bp 3'UTR, encoding 227 amino acids. Under hypoxia-reoxygen stress, the expression of Mn-SOD in brain tissue was significantly lower than in the control group after 8 h of reoxygenation and higher than the control group after 24 h. Hypoxia and subsequent reoxygenation triggered a disturbance in antioxidant homeostasis, displayed in the modification of GPx expression/activity in the liver: GPx was improved.
These results provide valuable information on the role of Mn-SOD regulation in oxidative stress caused by hypoxia.
环境缺氧会影响生物的生存和发育。它也是导致氧化损伤的重要环境因素。缺氧是组织缺氧的状态；再氧合是缺氧组织暴露于氧气的现象。缺氧-再氧合在发病机制中至关重要，其中活性氧的产生和抗氧化剂的差异对疾病的进展有显著贡献，并且是水产养殖行业中最常见的生理应激之一。
在这项研究中，使用快速扩增 cDNA 末端分析了健康军曹鱼（Rachycentron canadum）锰超氧化物歧化酶（Mn-SOD）基因的全长 cDNA。实时定量聚合酶链反应用于测量 Mn-SOD mRNAs 在各种组织（心脏、肌肉、大脑、肝脏、肾脏、鳃、肠和脾脏）中的表达水平。使用 2 法进行表达分析。使用 SPSS ver. 19.0（https://spss.software.informer.com/19.0/）分析实验数据。设 P<0.05 和 P<0.01 为差异有统计学意义。值表示为平均值±标准差。Mn-SOD 基因 cDNA 序列长 1209bp，包括 684bp 开放阅读框、42bp 5'UTR 和 483bp 3'UTR，编码 227 个氨基酸。在缺氧-再氧应激下，脑组织中 Mn-SOD 的表达在再氧合 8h 后明显低于对照组，在再氧合 24h 后高于对照组。缺氧和随后的再氧合触发了抗氧化剂动态平衡的紊乱，表现在肝脏中 GPx 表达/活性的改变：GPx 得到改善。
这些结果为 Mn-SOD 调节在缺氧引起的氧化应激中的作用提供了有价值的信息。
