Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean University, Lianyungang, 222005, China.
Fish Physiol Biochem. 2023 Dec;49(6):1171-1185. doi: 10.1007/s10695-023-01249-7. Epub 2023 Oct 13.
Avermectin is widely used as an important insecticide in agricultural production, but it also shows strong toxicity to non-target organisms. Quercetin is a natural flavonoid that is widely used due to its good anti-inflammatory and antioxidant effects. We believe that quercetin may have a potential therapeutic effect on avermectin poisoning. This experiment was proposed to observe the effect of quercetin on the toxic response to avermectin by observing the toxic response caused by avermectin in the brain of carp. In this project, 60 carp were studied as control group (Control), quercetin administration group (QUE), avermectin exposure group (AVM) and quercetin treatment avermectin exposure group (QUE + AVM) with different interventions to study the effect of quercetin on avermectin. The carp brain tissues were stained and simultaneously analyzed for blood-brain barrier (BBB), oxidative stress indicators, inflammatory factors, and apoptosis using qPCR technique. The results of the study indicate that avermectin exhibits a neurotoxic mechanism of action in fish by decreasing the transcript levels of tight junction protein-related genes, which in turn leads to the rupture of the BBB in the carp brain tissue. Avermectin induced apoptosis in carp brain tissue by increasing oxidative stress response and promoting inflammatory cell infiltration. Quercetin could reduce the accumulation of reactive oxygen species (ROS) in the brain tissue of carp caused by avermectin exposure toxicity, maintain redox homeostasis, reduce inflammatory response, and protect brain tissue cells from apoptosis. The present study confirmed the therapeutic and protective effects of quercetin on neurotoxicity in carp caused by avermectin exposure.
阿维菌素作为农业生产中一种重要的杀虫剂被广泛应用，但它对非靶标生物也表现出很强的毒性。槲皮素是一种天然类黄酮，由于其良好的抗炎和抗氧化作用而被广泛应用。我们认为槲皮素可能对阿维菌素中毒具有潜在的治疗作用。本实验通过观察阿维菌素对鲤鱼大脑中引起的毒性反应，提出观察槲皮素对阿维菌素毒性反应的影响。在这个项目中，以 60 条鲤鱼作为对照组（Control）、槲皮素给药组（QUE）、阿维菌素暴露组（AVM）和槲皮素处理阿维菌素暴露组（QUE+AVM）进行不同干预，研究槲皮素对阿维菌素的影响。采用 qPCR 技术对鲤鱼脑组织进行染色，同时分析血脑屏障（BBB）、氧化应激指标、炎症因子和细胞凋亡。研究结果表明，阿维菌素通过降低紧密连接蛋白相关基因的转录水平，对鱼类表现出神经毒性作用机制，进而导致鲤鱼脑组织中 BBB 的破裂。阿维菌素通过增加氧化应激反应和促进炎症细胞浸润，诱导鲤鱼脑组织细胞凋亡。槲皮素可减少阿维菌素暴露毒性引起的鲤鱼脑组织中活性氧（ROS）的积累，维持氧化还原平衡，减少炎症反应，保护脑组织细胞免受凋亡。本研究证实了槲皮素对阿维菌素暴露引起的鲤鱼神经毒性的治疗和保护作用。
