用于生产硫酸化多糖的紫球藻半连续固定化培养
Semi-continuous immobilized cultivation of Porphyridium cruentum for sulfated polysaccharides production.
作者信息
Han Sang-Il, Jeon Min Seo, Park Yun Hwan, Kim Sok, Choi Yoon-E
机构信息
Institute of Green Manufacturing Technology, Korea University, Seoul 02841, Republic of Korea; Division of Environmental Science & Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.
Division of Environmental Science & Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.
出版信息
Bioresour Technol. 2021 Dec;341:125816. doi: 10.1016/j.biortech.2021.125816. Epub 2021 Aug 21.
In this study, semi-continuous immobilized cultivation of Porphyridium cruentum through calcium alginate beads was performed for sulfated polysaccharides (SPs) production. The cell biomass and daily SPs productivity in the calcium alginate bead immobilized culture were increased by up to 79 ± 3.4% and 45.6 ± 3.2%, compared to those in the control, respectively. Furthermore, simultaneous application of immobilization and blue wavelength illumination further increased the phycobiliproteins content by 260 ± 9%, compared to those in the control. Similarly, nutrient deficiencies in combination with immobilization increased daily SPs productivity by about twice that of the control. The chemical composition and biological activity of the extracellular polymeric substances produced through immobilization were similar to those of the control. This study suggests the potential application of calcium alginate beads-based immobilization for continuous and high-efficiency SPs production using P. cruentum.
在本研究中，通过海藻酸钙珠对紫球藻进行半连续固定化培养以生产硫酸化多糖（SPs）。与对照相比，海藻酸钙珠固定化培养中的细胞生物量和每日SPs生产力分别提高了79±3.4%和45.6±3.2%。此外，与对照相比，固定化与蓝光照射同时应用使藻胆蛋白含量进一步提高了260±9%。同样，营养缺乏与固定化相结合使每日SPs生产力提高到对照的约两倍。通过固定化产生的细胞外聚合物的化学成分和生物活性与对照相似。本研究表明基于海藻酸钙珠的固定化在利用紫球藻连续高效生产SPs方面具有潜在应用价值。
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