Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC-UAM), Calle Nicolás Cabrera 9, 28049 Madrid, Spain.
Molecules. 2019 Apr 20;24(8):1564. doi: 10.3390/molecules24081564.
As the interest in biorefinery approaches is continuously increasing, new alternatives for the downstream valorization of biomasses are sought. microalga is a good natural source for a variety of interesting bioactive compounds, including carotenoids, phycoerythrin, and sulfated polysaccharides. In the present contribution, the use of compressed fluids-based techniques is explored towards the efficient and green extraction of bioactive compounds to valorize microalgal biomass. The extraction of carotenoids was first optimized using pressurized ethanol. The best extraction conditions involved the use of 125 °C for 20 min at 10.5 MPa. Subsequently, a sequential valorization process was devised based on the application of different steps directed towards the extraction of phycoerythrin, sulfated polysaccharides, and carotenoids, respectively. The applied pressurized conditions allowed the attainment of a good recovery of polar components without compromising the stability and extraction of carotenoids. Therefore, the proposed approach could be employed to obtain different bioactives from microalgal biomass employing green extraction processes.
随着人们对生物炼制的兴趣不断增加，人们正在寻找新的方法来对生物质进行下游增值利用。微藻是各种有价值的生物活性化合物的良好天然来源，包括类胡萝卜素、藻红蛋白和硫酸多糖。在本研究中，探索了基于压缩流体的技术在高效和绿色提取生物活性化合物以增值微藻生物质方面的应用。首先使用加压乙醇对类胡萝卜素进行了优化提取。最佳提取条件为在 10.5 MPa 下 125°C 加热 20 分钟。随后，根据应用不同步骤分别提取藻红蛋白、硫酸多糖和类胡萝卜素的要求，设计了一个连续增值工艺。所采用的加压条件允许在不损害类胡萝卜素稳定性和提取的情况下，实现极性成分的良好回收。因此，该方法可用于使用绿色提取工艺从微藻生物质中获得不同的生物活性物质。
