Instituto de Biotecnología y Desarrollo Azul (IBYDA), Departamento de Ecología y Geología, Facultad de Ciencias, Universidad de Málaga, Facultad de Ciencias, 29071 Málaga, Spain.
Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Regional Universitario de Málaga, Universidad de Málaga, 29071 Málaga, Spain.
Biomolecules. 2021 Mar 24;11(4):488. doi: 10.3390/biom11040488.
is a unicellular microalga that can synthesize and secrete to the culture medium-high amounts of polysaccharides. In this study, the immunomodulatory, cytotoxic effect and antioxidant activity of the sulfated polysaccharides (PcSPs) were determinate. The PcSPs were precipitated with 2% Cetylpyridinium bromide hydrate and ethanol and purified by dialysis. The extract was lyophilized for its characterization by Fourier transform-Infrared (FT-IR) spectroscopy and gas chromatography-mass spectrometry (GC-MS). The antioxidant activity of PcSPs were examined with assay 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and compared with that of the biomass, observing significant differences between the results obtained from the PcSPs and biomass. To determine their ability to induce cytokine production Tumor Necrosis Factor alpha (TNF-α) and interleukina-6 (IL-6), the immunomodulatory activity of the PcSPs has been evaluated. In the mouse macrophage cell line (RAW 264.7), PcSPs are potent inducers of IL-6 cytokines but mainly of TNF-α. The cytotoxic capacity of PcSPs was measured by the MTT colorimetric assay in colorectal carcinoma (HTC-116), human leukemia (U-937 and HL-60), breast cancer (MCF-7), lung cancer (NCI-H460) and human gingival fibroblasts (HGF-1) cell lines. The IC value of 2311.20 µg mL, 1676.74 µg mL, 1089.63 µg mL, 5498.14 µg mL and 2861.49 µg mL respectively in the tumor lines and 5022.55 µg mL in gingival fibroblasts were obtained. Our study suggested that PcSPs from have a moderate immunomodulatory and cytotoxic effect. The results obtained indicate that the polysaccharides from are potent inducers of IL-6 cytokines and, most importantly, of TNF-α. PcSPs showed no evidence of antigenic activity or hypersensitivity when administered intraperitoneally in mice. Furthermore, the in vivo study revealed an improvement of local inflammatory response against stress in the peritoneum. These findings suggest that the PcSPs from might have potential as a valuable ingredient in nutraceutical products.
是一种单细胞微藻，能够向培养基中合成和分泌大量多糖。在这项研究中，测定了硫酸化多糖（PcSPs）的免疫调节、细胞毒性作用和抗氧化活性。PcSPs 用 2%溴化十六烷基吡啶水和乙醇沉淀，然后通过透析进行纯化。提取物经冷冻干燥后，用傅里叶变换红外（FT-IR）光谱和气相色谱-质谱（GC-MS）进行表征。通过 2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）测定 PcSPs 的抗氧化活性，并与生物质的结果进行比较，观察到 PcSPs 和生物质的结果之间存在显著差异。为了确定它们诱导肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）产生的能力，评估了 PcSPs 的免疫调节活性。在小鼠巨噬细胞系（RAW 264.7）中，PcSPs 是细胞因子 IL-6 的有效诱导剂，但主要是 TNF-α。通过 MTT 比色法在结直肠癌细胞系（HTC-116）、人白血病细胞系（U-937 和 HL-60）、乳腺癌细胞系（MCF-7）、肺癌细胞系（NCI-H460）和人牙龈成纤维细胞系（HGF-1）中测定 PcSPs 的细胞毒性。在肿瘤细胞系中，IC 值分别为 2311.20 µg/mL、1676.74 µg/mL、1089.63 µg/mL、5498.14 µg/mL 和 2861.49 µg/mL，在牙龈成纤维细胞中为 5022.55 µg/mL。我们的研究表明，来自 的 PcSPs 具有适度的免疫调节和细胞毒性作用。研究结果表明，来自 的多糖是细胞因子 IL-6 的有效诱导剂，最重要的是 TNF-α。PcSPs 在小鼠腹腔内给药时没有表现出抗原性或过敏反应的证据。此外，体内研究显示，腹腔内局部炎症反应对应激有改善作用。这些发现表明，来自 的 PcSPs 可能具有作为营养保健品中一种有价值成分的潜力。
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