Pharmacy Department of Beijing Chao-Yang Hospital Affiliated with Beijing Capital Medical University, Beijing, 100020, PR China.
Department of Clinical Laboratory, Peking Union Medical College Hospital, China Academic Medical Science and Peking Union Medical College, Beijing, 100730, PR China.
Clin Nutr. 2021 Sep;40(9):5053-5061. doi: 10.1016/j.clnu.2021.01.012. Epub 2021 Jan 23.
Pregnancy generally alters the balance of maternal metabolism, but the molecular profiles in early pregnancy and associated factors of folate supplementation in pregnant women remains incompletely understood.
Untargeted metabonomics based on high-performance liquid chromatography-high-resolution mass spectrometry integrated with multivariate metabolic pathway analysis were applied to characterize metabolite profiles and associated factors of folate supplements in early pregnancy. The metabolic baseline of early pregnancy was determined by metabolic analysis of 510 serum samples from 131 non-pregnant and 379 pregnant healthy Chinese women. The pathophysiology of adaptive reactions and metabolic challenges induced by folate supplementation in early pregnancy was further compared between pregnant women with (n = 168) and without (n = 184) folate supplements.
Compared with non-pregnant participants, 106 metabolites, majority of which are related to amino acids and lysophosphatidylcholine/phosphatidylcholine, and 13 metabolic pathways were significantly changed in early pregnancy. The supplementation of folate in early pregnancy induced marked changes in N-acyl ethanolamine 22:0, N-acyl taurine 18:2, glycerophosphoserine 44:1 and 8,11,14-eicosatrienoate, proline, and aminoimidazole ribotide levels.
During early pregnancy, the metabolism of amino acids significantly changes to meet the physiological requirements of pregnant women. Folate intake may change glucose and lipid metabolism. These findings provide a comprehensive landscape for understanding the basic characteristics and gestational metabolic networks of early pregnancy and folate supplementation. This study provides a basis for further research into the relationship between metabolic markers and pregnancy diseases.
This study protocol was registered on www.ClinicalTrials.gov, NCT03651934, on August 29, 2018 (prior to recruitment).
妊娠通常会改变母体代谢的平衡，但人们对妊娠早期的分子谱以及孕妇叶酸补充的相关因素仍了解不完全。
本研究采用基于高效液相色谱-高分辨率质谱联用的靶向代谢组学方法，并结合多元代谢途径分析，以描绘妊娠早期叶酸补充的代谢物谱及其相关因素。通过对 131 名非妊娠和 379 名健康中国妊娠妇女的 510 份血清样本进行代谢分析，确定妊娠早期的代谢基线。进一步比较了妊娠妇女（n=168）和非妊娠妇女（n=184）中补充叶酸前后的适应性反应和代谢挑战的病理生理学差异。
与非妊娠参与者相比，106 种代谢物（其中大多数与氨基酸和溶血磷脂酰胆碱/磷脂酰胆碱有关）和 13 条代谢途径在妊娠早期发生了显著变化。妊娠早期补充叶酸会导致 N-酰基乙醇胺 22:0、N-酰基牛磺酸 18:2、甘油磷酸丝氨酸 44:1 和 8,11,14-二十碳三烯酸、脯氨酸和氨基咪唑核糖苷水平发生明显变化。
在妊娠早期，氨基酸代谢显著改变以满足孕妇的生理需求。叶酸的摄入可能会改变葡萄糖和脂质代谢。这些发现为了解妊娠早期和叶酸补充的基本特征和妊娠代谢网络提供了全面的认识。本研究为进一步研究代谢标志物与妊娠疾病的关系提供了依据。
本研究方案于 2018 年 8 月 29 日在 www.ClinicalTrials.gov 上注册，注册号为 NCT03651934（在招募前）。
