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高效液相色谱法对手性药物活性物质的分离概述（2018 - 2020年）

An overview of chiral separations of pharmaceutically active substances by HPLC (2018-2020).

作者信息

Grybinik Sofiya, Bosakova Zuzana

机构信息

Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic.

出版信息

Monatsh Chem. 2021;152(9):1033-1043. doi: 10.1007/s00706-021-02832-5. Epub 2021 Aug 24.

DOI:10.1007/s00706-021-02832-5
PMID:34456367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8382579/
Abstract

This review provides a brief survey of chiral separation of pharmaceutically active substances published over the last 3 years (2018-2020). Chiral separation of drugs is an important area of research. The control of enantiomeric purity and determination of individual enantiomeric drug molecules is a necessity especially for clinical, analytical, and regulatory purposes. Among chromatographic resolution methods, high-performance liquid chromatography based on chiral stationary phases remains the most popular and effective method used for chiral separation of various drugs. In this review, attention is paid to several classes of chiral stationary phases that have been the most frequently used for drug enantioseparation during this period.

摘要

本综述简要概述了过去三年（2018 - 2020年）发表的关于药用活性物质的手性分离研究。药物的手性分离是一个重要的研究领域。对映体纯度的控制以及单个对映体药物分子的测定尤为必要，特别是出于临床、分析和监管目的。在色谱拆分方法中，基于手性固定相的高效液相色谱仍然是用于各种药物手性分离的最常用且有效的方法。在本综述中，重点关注了在此期间最常用于药物对映体拆分的几类手性固定相。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/99da865e5295/706_2021_2832_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/e348efeb2973/706_2021_2832_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/21318346297d/706_2021_2832_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/99da865e5295/706_2021_2832_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/e348efeb2973/706_2021_2832_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/21318346297d/706_2021_2832_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/56a4/8382579/99da865e5295/706_2021_2832_Fig3_HTML.jpg

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3
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