Mottaghi Soheil, Kohl Sandra, Biemann Dirk, Liebana Samuel, Montaño Crespo Ruth Eneida, Buchholz Oliver, Wilson Mareike, Klaus Carolin, Uchenik Michelle, Münkel Christian, Schmidt Robert, Hofmann Ulrich G
Neuroelectronic Systems, Department of Neurosurgery, Medical Center, University of Freiburg, Freiburg, Germany.
Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Front Neurosci. 2021 Aug 12;15:700672. doi: 10.3389/fnins.2021.700672. eCollection 2021.
Cortico-basal ganglia beta oscillations (13-30 Hz) are assumed to be involved in motor impairments in Parkinson's Disease (PD), especially in bradykinesia and rigidity. Various studies have utilized the unilateral 6-hydroxydopamine (6-OHDA) rat PD model to further investigate PD and test novel treatments. However, a detailed behavioral and electrophysiological characterization of the model, including analyses of popular PD treatments such as DBS, has not been documented in the literature. We hence challenged the 6-OHDA rat hemi-PD model with a series of experiments (i.e., cylinder test, open field test, and rotarod test) aimed at assessing the motor impairments, analyzing the effects of Deep Brain Stimulation (DBS), and identifying under which conditions excessive beta oscillations occur. We found that 6-OHDA hemi-PD rats presented an impaired performance in all experiments compared to the sham group, and DBS could improve their overall performance. Across all the experiments and behaviors, the power in the high beta band was observed to be an important biomarker for PD as it showed differences between healthy and lesioned hemispheres and between 6-OHDA-lesioned and sham rats. This all shows that the 6-OHDA hemi-PD model accurately represents many of the motor and electrophysiological symptoms of PD and makes it a useful tool for the pre-clinical testing of new treatments when low β (13-21 Hz) and high β (21-30 Hz) frequency bands are considered separately.
皮质-基底神经节β振荡（13 - 30赫兹）被认为与帕金森病（PD）的运动障碍有关，尤其是在运动迟缓及僵硬方面。各种研究利用单侧6-羟基多巴胺（6-OHDA）大鼠帕金森病模型来进一步研究帕金森病并测试新的治疗方法。然而，该模型详细的行为和电生理特征，包括对诸如脑深部电刺激（DBS）等常用帕金森病治疗方法的分析，在文献中尚未有记载。因此，我们通过一系列实验（即圆筒实验、旷场实验和转棒实验）对6-OHDA大鼠半侧帕金森病模型进行了研究，旨在评估运动障碍、分析脑深部电刺激（DBS）的效果，并确定在何种条件下会出现过度的β振荡。我们发现，与假手术组相比，6-OHDA半侧帕金森病大鼠在所有实验中表现受损，而脑深部电刺激（DBS）可改善其整体表现。在所有实验和行为中，高β频段的功率被观察到是帕金森病的一个重要生物标志物，因为它在健康半球和受损半球之间以及6-OHDA损伤大鼠和假手术大鼠之间显示出差异。这一切表明，6-OHDA半侧帕金森病模型准确地反映了帕金森病的许多运动和电生理症状，当分别考虑低β（13 - 21赫兹）和高β（21 - 30赫兹）频段时，它成为新治疗方法临床前测试的有用工具。
