Liu Jiayi, Hua Juan, Qu Bo, Guo Xuanyue, Wang Yangyang, Shao Meini, Luo Shihong
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Key Laboratory of Biological Invasions and Global Changes, Shenyang, China.
Front Plant Sci. 2021 Aug 12;12:720816. doi: 10.3389/fpls.2021.720816. eCollection 2021.
Essential oils (EOs) are often the source of insecticidal substances of high efficiency and low toxicity. From gas chromatograph-mass spectrometer, column chromatography, and nuclear magnetic resonance spectra analyses, twenty terpenes were identified from the EOs of . These comprised mostly monoterpenes (49.01%) and sesquiterpenes (50.76%). The terpenes at the highest concentrations in the EOs of were feropodin (200.46 ± 1.42 μg/ml), (+)-camphor (154.93 ± 9.72 μg/ml), β-selinene (57.73 ± 2.48 μg/ml), and 1,8-cineole (17.99 ± 1.06 μg/ml), calculated using area normalization and external standards. The EOs were tested for biological activity and showed strong fumigant toxicity and significant antifeedant activity against the larvae of . Furthermore, the monoterpenes 1,8-cineole and (+)-camphor displayed significant fumigant activity against , with LC values of 7.00 ± 0.85 and 18.16 ± 2.31 μl/L, respectively. Antifeedant activity of the sesquiterpenes feropodin and β-selinene was obvious, with EC values of 12.23 ± 2.60 and 10.46 ± 0.27 μg/cm, respectively. The EOs and β-selinene were also found to inhibit acetylcholinesterase, with IC values of 37.75 ± 3.59 and 6.88 ± 0.48 μg/ml, respectively. These results suggest that monoterpenes and sesquiterpenes from the EOs of could potentially be applied as a botanical pesticides in the control of .
精油（EOs）通常是高效低毒杀虫物质的来源。通过气相色谱 - 质谱仪、柱色谱和核磁共振光谱分析，从[具体植物名称未给出]的精油中鉴定出了20种萜类化合物。这些萜类化合物主要包括单萜（49.01%）和倍半萜（50.76%）。采用面积归一化法和外标法计算得出，[具体植物名称未给出]精油中含量最高的萜类化合物为feropodin（200.46 ± 1.42 μg/ml）、（+）-樟脑（154.93 ± 9.72 μg/ml）、β-石竹烯（57.73 ± 2.48 μg/ml）和1,8-桉叶素（17.99 ± 1.06 μg/ml）。对这些精油进行了生物活性测试，结果表明它们对[具体害虫名称未给出]的幼虫具有很强的熏蒸毒性和显著的拒食活性。此外，单萜类化合物1,8-桉叶素和（+）-樟脑对[具体害虫名称未给出]表现出显著的熏蒸活性，其LC值分别为7.00 ± 0.85和18.16 ± 2.31 μl/L。倍半萜类化合物feropodin和β-石竹烯的拒食活性明显，其EC值分别为12.23 ± 2.60和10.46 ± 0.27 μg/cm。还发现这些精油和β-石竹烯能够抑制乙酰胆碱酯酶，其IC值分别为37.75 ± 3.59和6.88 ± 0.48 μg/ml。这些结果表明，[具体植物名称未给出]精油中的单萜和倍半萜有可能作为植物源农药用于防治[具体害虫名称未给出]。
