Cabaj Władysław, Grzelak Sylwia, Moskwa Bożena, Bień-Kalinowska Justyna
The Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warsaw, Poland.
Int J Parasitol Parasites Wildl. 2021 Jul 31;16:59-63. doi: 10.1016/j.ijppaw.2021.07.010. eCollection 2021 Dec.
European bison are susceptible to a range of pathogens which may influence their health, and hence, to ensure their protection, it is essential to provide effective monitoring of potential exposure. This study presents the first molecular confirmation of infection in European bison based on PCR amplification of the cytochrome oxidase subunit I () gene. A sample of heart tissue taken from one fifteen-year-old European bison cow was examined by light microscopy for the presence of heart sarcocysts. The genomic DNA isolated from any identified sarcocysts was subjected to PCR to amplify gene sequences, and the obtained amplicons were sequenced by Sanger dideoxy sequencing. Two partial sequences were obtained; they were identified as and deposited in the GenBank™ database under the accession numbers MW490605 and MW490606. BLAST analysis found them to demonstrate the closest similarity to (MH255771-MH255779 and KU247874-KU247884), sharing an identity of 93.14-93.8 %. This is the first report to identify sarcocysts isolated from heart tissue of infected European bison living in the Białowieża forest to species level using analysis. Our findings confirm that the European bison is a natural intermediate host for . As such, coordinators of future conservation programmes should consider the impact of these diseases on reintroduced animals.
欧洲野牛易受一系列可能影响其健康的病原体感染，因此，为确保对它们的保护，对潜在接触情况进行有效监测至关重要。本研究基于细胞色素氧化酶亚基I（）基因的PCR扩增，首次对欧洲野牛感染情况进行了分子确认。从一头15岁的欧洲野牛母牛身上采集心脏组织样本，通过光学显微镜检查是否存在心脏肉孢子虫。从任何鉴定出的肉孢子虫中分离出基因组DNA，进行PCR扩增基因序列，并通过桑格双脱氧测序法对获得的扩增子进行测序。获得了两个部分序列；它们被鉴定为，并以登录号MW490605和MW490606保存在GenBank™数据库中。BLAST分析发现它们与（MH255771 - MH255779和KU247874 - KU247884）的相似度最高，同一性为93.14 - 93.8%。这是第一份利用分析将从生活在比亚沃维耶扎森林中受感染欧洲野牛心脏组织分离出的肉孢子虫鉴定到物种水平的报告。我们的研究结果证实欧洲野牛是 的天然中间宿主。因此，未来保护计划的协调者应考虑这些疾病对重新引入动物的影响。
