Calero-Bernal Rafael, Verma Shiv K, Seaton C Tom, Sinnett David, Ball Erin, Dunams Detiger, Rosenthal Benjamin M, Dubey Jitender P
Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville 20705-2350, MD, USA.
Alaska Department of Fish and Game, Fairbanks, AK 99701, USA.
Vet Parasitol. 2015 May 30;210(1-2):102-5. doi: 10.1016/j.vetpar.2015.03.007. Epub 2015 Mar 20.
Endangered wood bison (Bison bison athabascae) is the largest terrestrial mammal in the American continent. Animal health is an important issue in their conservation, and Sarcocystis cruzi may be a cause of clinical disease in Bovidae. Hearts of eight wood bison from Alaska, USA were examined for sarcocysts by histology, transmission electron microscopy, pepsin digestion, and molecularly. Sarcocystis bradyzoites were found in pepsin digests of all eight and sarcocysts were found in histologic sections of myocardium of four bison. Sarcocysts were thin-walled and ultrastructurally consistent with S. cruzi. Characterization of DNA obtained from lysis of pepsin liberated bradyzoites by PCR-RFLP and subsequent phylogenetic analyses matched with that previously reported for S. cruzi infecting cattle in the USA. Collectively, data indicate that wood bison is a natural intermediate host for S. cruzi.
濒危的林地美洲野牛（Bison bison athabascae）是美洲大陆最大的陆生哺乳动物。动物健康是其保护中的一个重要问题，而克鲁斯肉孢子虫可能是牛科动物临床疾病的一个病因。对来自美国阿拉斯加的八头林地美洲野牛的心脏进行了组织学、透射电子显微镜检查、胃蛋白酶消化和分子检测，以查找肉孢子虫。在所有八头野牛的胃蛋白酶消化物中均发现了肉孢子虫缓殖子，在四头野牛心肌的组织切片中发现了肉孢子囊。肉孢子囊壁薄，超微结构与克鲁斯肉孢子虫一致。通过PCR-RFLP对胃蛋白酶释放的缓殖子裂解后获得的DNA进行表征，并进行后续系统发育分析，结果与之前报道的感染美国牛的克鲁斯肉孢子虫相匹配。总体而言，数据表明林地美洲野牛是克鲁斯肉孢子虫的天然中间宿主。
