Zhou Xingang, Liang Tingyu, Deng Jinhai, Ng Kenrick, Li Man, Lv Chunxin, Chen Jiamin, Yang Kun, Ma Zhiyuan, Ma Wenping, Wang Peng
Department of Pathology, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
Department of Neurosurgery, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
Front Cell Dev Biol. 2021 Aug 11;9:697086. doi: 10.3389/fcell.2021.697086. eCollection 2021.
Diffuse glioma is the most common primary tumor of the central nervous system. The prognosis of the individual tumor is heavily dependent on its grade and subtype. Homeobox B7 (HOXB7), a member of the homeobox family, is abnormally overexpressed in a variety of tumors. However, its function in glioma is unclear. In this study, HOXB7 mRNA and protein expression levels were analyzed in 401 gliomas from the CGGA RNA-seq database (325 cases) and our hospital (76 cases). HOXB7 expression, at both mRNA and protein levels, were upregulated in glioblastoma (GBM) and isocitrate dehydrogenase 1 () wild-type glioma tissues. Kaplan-Meier with log-rank test showed that patients with high HOXB7 expression had a poor prognosis ( < 0.0001). Moreover, HOXB7 protein was deleted in 90.9% (20/22) of oligodendrogliomas and 13.0% (3/23) of astrocytomas. The sensitivity and specificity of HOXB7 protein deletion in oligodendroglioma were 90.9% (20/22) and 87.0% (20/23), respectively. To verify the reliability of using HOXB7 in differentiating oligodendroglioma, we used 1p/19q fluorescence hybridization (FISH) testing as a positive control. The Cohen's kappa coefficient of HOXB7 immunohistochemistry staining and 1p/19q FISH testing was 0.778 (95% CI: 0.594-0.962, < 0.001). In conclusion, HOXB7 is an independent predictor of poor prognosis in all grade gliomas. Additionally, HOXB7 is also a highly sensitive and specific indicator to differentiate oligodendroglioma from astrocytoma.
弥漫性胶质瘤是中枢神经系统最常见的原发性肿瘤。单个肿瘤的预后很大程度上取决于其分级和亚型。同源盒B7（HOXB7）是同源盒家族的成员之一，在多种肿瘤中异常高表达。然而，其在胶质瘤中的功能尚不清楚。在本研究中，对来自CGGA RNA测序数据库（325例）和我院（76例）的401例胶质瘤进行了HOXB7 mRNA和蛋白表达水平分析。HOXB7在mRNA和蛋白水平的表达在胶质母细胞瘤（GBM）和异柠檬酸脱氢酶1（IDH1）野生型胶质瘤组织中均上调。Kaplan-Meier法结合对数秩检验显示，HOXB7高表达患者预后较差（P<0.0001）。此外，90.9%（20/22）的少突胶质细胞瘤和13.0%（3/23）的星形细胞瘤中HOXB7蛋白缺失。HOXB7蛋白缺失在少突胶质细胞瘤中的敏感性和特异性分别为90.9%（20/22）和87.0%（20/23）。为验证使用HOXB7鉴别少突胶质细胞瘤的可靠性，我们采用1p/19q荧光原位杂交（FISH）检测作为阳性对照。HOXB7免疫组化染色与1p/19q FISH检测的Cohen's kappa系数为0.778（95%CI：0.594-0.962，P<0.001）。总之，HOXB7是所有级别胶质瘤预后不良的独立预测因子。此外，HOXB7也是鉴别少突胶质细胞瘤和星形细胞瘤的高度敏感和特异的指标。
