Kim Donghyun, Yoon Yeo-Jun, Choi Dojin, Kim Jisun, Lim Jae-Yol
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, South Korea.
Front Cell Dev Biol. 2021 Aug 12;9:698292. doi: 10.3389/fcell.2021.698292. eCollection 2021.
Lumen formation of salivary glands has been investigated using or rudiment culture models. In this study, we used a three-dimensional (3D) salivary gland organoid culture system and demonstrated that lumen formation could be recapitulated in mouse SMG organoids. In our organoid culture system, lumen formation was induced by vasoactive intestinal peptide and accelerated by treatment with RA. Furthermore, lumen formation was observed in branching duct-like structure when cultured in combination of fibroblast growth factors (FGF) in the presence of retinoic acid (RA). We suggest RA signaling-mediated regulation of VIPR1 and KRT7 as the underlying mechanism for lumen formation, rather than apoptosis in the organoid culture system. Collectively, our results support a fundamental role for RA in lumen formation and demonstrate the feasibility of 3D organoid culture as a tool for studying salivary gland morphogenesis.
唾液腺管腔形成已通过使用[具体内容缺失]或原基培养模型进行了研究。在本研究中，我们使用了三维（3D）唾液腺类器官培养系统，并证明在小鼠下颌下腺类器官中可以重现管腔形成。在我们的类器官培养系统中，血管活性肠肽诱导管腔形成，视黄酸（RA）处理加速管腔形成。此外，当在成纤维细胞生长因子（FGF）与视黄酸（RA）共同存在的条件下培养时，在分支导管样结构中观察到了管腔形成。我们认为，在类器官培养系统中，视黄酸信号介导的VIPR1和KRT7调节是管腔形成的潜在机制，而非细胞凋亡。总体而言，我们的结果支持视黄酸在管腔形成中的重要作用，并证明了3D类器官培养作为研究唾液腺形态发生工具的可行性。
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