Hurcombe Samuel D, Roessner Holly A, Klein Chelsea E, Engiles Julie B, Hopster Klaus
Department of Clinical Sciences, School of Veterinary Medicine, New Bolton Center, University of Pennsylvania, Kennett Square, PA, United States.
Department of Pathobiology, School of Veterinary Medicine, New Bolton Center, University of Pennsylvania, Kennett Square, PA, United States.
Front Vet Sci. 2021 Aug 12;8:639424. doi: 10.3389/fvets.2021.639424. eCollection 2021.
Jejunal vascular ligation is an essential step in performing jejunojejunostomy. Hand sewn ligation is typically used and can increase operative time with long sections of bowel to be removed. Nylon cable ties (NCT) have been used for vascular ligation in horses but are yet to be investigated for application on the mesenteric vasculature of the gastrointestinal tract. Our objective was to evaluate the efficacy and short-term safety of NCT jejunal mesenteric vessel ligation in healthy horses. Eight healthy adult horses underwent midline celiotomy. A segment of jejunal mesentery was identified (≥4 arcades). Briefly, three fenestrations (proximal, middle, distal) were made 5-10 mm apart adjacent to the first and last vascular arcade to be ligated. Two sterilized NCT were passed to encircle the mesentery through the proximal and middle fenestrations, separated by intact mesentery. NCT were closed tightly and the vascular pedicle transected with Mayo scissors through the distal fenestration. Jejunojejunostomy was then performed and the mesentery sutured closed. The number of vascular arcades and time to ligate using NCT were recorded. At 2 weeks, horses underwent repeat celiotomy to assess the healing of the NCT ligation site and an equal number of vascular arcades were hand sewn double ligated using 2-0 Polyglactin 910 as a timed comparison. NCT mesenteric ligation was significantly faster than hand sewn methods ( < 0.01). Effective hemostasis was achieved in all cases. There was no evidence of local infection or adhesions at 14 days post-operatively. Further investigation in the long-term effects in horses as well as horses with strangulating jejunal lesions are needed for clinical application.
空肠血管结扎是进行空肠空肠吻合术的关键步骤。通常采用手工缝合结扎,对于需要切除较长肠段的情况,这会增加手术时间。尼龙扎带(NCT)已用于马匹的血管结扎,但尚未在胃肠道肠系膜血管上进行应用研究。我们的目的是评估NCT空肠系膜血管结扎在健康马匹中的疗效和短期安全性。八匹健康成年马接受了中线剖腹术。识别出一段空肠系膜(≥4个血管弓)。简要地说,在要结扎的第一个和最后一个血管弓相邻处,相隔5 - 10毫米制作三个开窗(近端、中间、远端)。将两根消毒的NCT穿过近端和中间开窗环绕肠系膜,中间由完整的肠系膜隔开。将NCT紧紧闭合,通过远端开窗用梅奥剪刀切断血管蒂。然后进行空肠空肠吻合术,并缝合肠系膜使其闭合。记录血管弓的数量和使用NCT结扎所需的时间。在2周时,马匹接受再次剖腹术以评估NCT结扎部位的愈合情况,并使用2 - 0聚乙醇酸910进行手工缝合双重结扎相同数量的血管弓作为定时对照。NCT肠系膜结扎明显比手工缝合方法快(<0.01)。所有病例均实现了有效止血。术后14天没有局部感染或粘连的迹象。对于临床应用,需要进一步研究其在马匹以及患有绞窄性空肠病变马匹中的长期影响。