来自脊髓背角的小鼠神经元原代培养
Primary Culture of Mouse Neurons from the Spinal Cord Dorsal Horn.
作者信息
Cao De-Li, Peng BoJing, Bao ChunJiang, Gao Yong-Jing
机构信息
Institute of Nautical Medicine, Nantong University, Jiangsu, China.
Co-innovation Center of Neuroregeneration, Nantong University, Jiangsu, China.
出版信息
Bio Protoc. 2017 Jan 5;7(1):e2098. doi: 10.21769/BioProtoc.2098.
Abstract
Primary afferents of sensory neurons mainly terminate in the spinal cord dorsal horn, which has an important role in the integration and modulation of sensory-related signals. Primary culture of mouse spinal dorsal horn neuron (SDHN) is useful for studying signal transmission from peripheral nervous system to the brain, as well as for developing cellular disease models, such as pain and itch. Because of the specific features of SDHN, it is necessary to establish a reliable culture method that is suitable for testing neural response to various external stimuli .
摘要
感觉神经元的初级传入纤维主要终止于脊髓背角，脊髓背角在感觉相关信号的整合与调制中起重要作用。小鼠脊髓背角神经元（SDHN）的原代培养对于研究从外周神经系统到大脑的信号传递以及开发细胞疾病模型（如疼痛和瘙痒）很有用。由于SDHN的特定特征，有必要建立一种可靠的培养方法，该方法适用于测试神经元对各种外部刺激的反应。
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