在无流动补料分批培养系统中富集嗜酸铁（II）氧化细菌。
Enriching Acidophilic Fe(II)-oxidizing Bacteria in No-flow, Fed-batch Systems.
作者信息
Sheng Yizhi, Kaley Bradley, Burgos William D
机构信息
Department of Civil and Environmental Engineering, the Pennsylvania State University, PA, USA.
School of Water Resources and Environment, China University of Geosciences, Beijing, China.
出版信息
Bio Protoc. 2017 Feb 5;7(3):e2130. doi: 10.21769/BioProtoc.2130.
Low-pH microbial Fe(II) oxidation occurs naturally in some Fe(II)-rich acid mine drainage (AMD) ecosystems across so-called terraced iron formations. Indigenous acidophilic Fe(II)-oxidizing bacterial communities can be incorporated into both passive and active treatments to remove Fe from the AMD solution. Here, we present a protocol of enriching acidophilic Fe(II)-oxidizing bacteria in no-flow, fed-batch systems. Mixed cultures of naturally occurring microbes are enriched from the fresh surface sediments at AMD sites using a chemo-static bioreactor (Eppendorf BioFlo/Celligen 115 Fermentor) with respect to constant stirring speed, temperature, pH and unlimited dissolved oxygen. Ferrous sulfate is discontinuously added to the reactor as the primary substrate to enrich for acidophilic Fe(II)-oxidizing bacteria. Successfully and efficiently enriching acidophilic Fe(II)-oxidizing bacteria helps to exploit this biogeochemical process into AMD treatment systems.
低pH值下的微生物铁（II）氧化在一些富含铁（II）的酸性矿山排水（AMD）生态系统中自然发生，这些生态系统分布在所谓的梯田状铁建造中。本地嗜酸铁（II）氧化细菌群落可被纳入被动和主动处理中，以从AMD溶液中去除铁。在此，我们展示了一种在无流动、补料分批系统中富集嗜酸铁（II）氧化细菌的方案。利用化学稳定生物反应器（艾本德BioFlo/Celligen 115发酵罐），在恒定搅拌速度、温度、pH值和无限溶解氧的条件下，从AMD场地的新鲜表层沉积物中富集天然存在的微生物混合培养物。硫酸亚铁作为主要底物间断添加到反应器中，以富集嗜酸铁（II）氧化细菌。成功且高效地富集嗜酸铁（II）氧化细菌有助于将这一生物地球化学过程应用于AMD处理系统。
Zotero 插件