Flajšman Marko, Radišek Sebastjan, Javornik Branka
Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Slovenian Institute of Hop Research and Brewing, Zalec, Slovenia.
Bio Protoc. 2017 Mar 20;7(6):e2171. doi: 10.21769/BioProtoc.2171.
is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant's xylem and causes wilt disease symptoms by secreting different virulence factors. Hop () is a primary host of , so it is used as a model plant for studying this phytopathogenic fungus. Artificial infections of hop plants and disease scoring are prerequisites for studying the pathogen's virulence/pathogenicity and its interaction with hop plants. In this protocol, we describe the root dipping inoculation method for conducting pathogenicity assay of on hop plants.
是一种土传植物病原体,通过根部感染宿主。它在植物的木质部中传播,并通过分泌不同的毒力因子引起枯萎病症状。啤酒花()是该病原体的主要宿主,因此它被用作研究这种植物病原真菌的模式植物。对啤酒花植株进行人工感染和病害评分是研究病原体毒力/致病性及其与啤酒花植株相互作用的先决条件。在本方案中,我们描述了用于对啤酒花植株进行致病性测定的浸根接种方法。