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Characterization of Verticillium albo-atrum Field Isolates Using Pathogenicity Data and AFLP Analysis.利用致病性数据和扩增片段长度多态性分析对黑白轮枝菌田间分离株进行特征分析
Plant Dis. 2003 Jun;87(6):633-638. doi: 10.1094/PDIS.2003.87.6.633.
2
Identification of Novel Virulence-Associated Proteins Secreted to Xylem by Verticillium nonalfalfae During Colonization of Hop Plants.在啤酒花植株定殖过程中，非苜蓿黄萎病菌分泌到木质部的新型毒力相关蛋白的鉴定
Mol Plant Microbe Interact. 2016 May;29(5):362-73. doi: 10.1094/MPMI-01-16-0016-R. Epub 2016 Apr 6.
3
Different Gene Expressions of Resistant and Susceptible Hop Cultivars in Response to Infection with a Highly Aggressive Strain of .抗性和敏感啤酒花品种对高侵袭性菌株感染的不同基因表达
Plant Mol Biol Report. 2015;33(3):689-704. doi: 10.1007/s11105-014-0767-4.
4
Comparative proteomic profiling in compatible and incompatible interactions between hop roots and Verticillium albo-atrum.Hop 根与黄萎轮枝菌亲和和非亲和互作中的比较蛋白质组分析。
Plant Physiol Biochem. 2013 Jul;68:23-31. doi: 10.1016/j.plaphy.2013.03.017. Epub 2013 Apr 8.
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Identification of quantitative trait loci for resistance to Verticillium wilt and yield parameters in hop (Humulus lupulus L.).鉴定啤酒花（Humulus lupulus L.）抗黄萎病和产量参数的数量性状位点。
Theor Appl Genet. 2013 Jun;126(6):1431-43. doi: 10.1007/s00122-013-2062-4. Epub 2013 Feb 20.
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The area under the disease progress stairs: calculation, advantage, and application.疾病进展阶梯下面积：计算、优势及应用。
Phytopathology. 2012 Apr;102(4):381-9. doi: 10.1094/PHYTO-07-11-0216.
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Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens.比较基因组学揭示了植物维管束萎蔫病原菌适应小生境的机制。
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Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1.番茄Ve1介导的黄萎病抗性的遗传剖析
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RNA silencing is required for Arabidopsis defence against Verticillium wilt disease.RNA沉默对于拟南芥抵御黄萎病是必需的。
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对啤酒花植株的致病性测定

Pathogenicity Assay of on Hop Plants.

作者信息

Flajšman Marko, Radišek Sebastjan, Javornik Branka

机构信息

Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

Slovenian Institute of Hop Research and Brewing, Zalec, Slovenia.

出版信息

Bio Protoc. 2017 Mar 20;7(6):e2171. doi: 10.21769/BioProtoc.2171.

DOI:10.21769/BioProtoc.2171
PMID:34458482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8376619/
Abstract

is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant's xylem and causes wilt disease symptoms by secreting different virulence factors. Hop () is a primary host of , so it is used as a model plant for studying this phytopathogenic fungus. Artificial infections of hop plants and disease scoring are prerequisites for studying the pathogen's virulence/pathogenicity and its interaction with hop plants. In this protocol, we describe the root dipping inoculation method for conducting pathogenicity assay of on hop plants.

摘要

是一种土传植物病原体，通过根部感染宿主。它在植物的木质部中传播，并通过分泌不同的毒力因子引起枯萎病症状。啤酒花（）是该病原体的主要宿主，因此它被用作研究这种植物病原真菌的模式植物。对啤酒花植株进行人工感染和病害评分是研究病原体毒力/致病性及其与啤酒花植株相互作用的先决条件。在本方案中，我们描述了用于对啤酒花植株进行致病性测定的浸根接种方法。