Wang Qing, Gottwald Sven
Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Giessen, Germany.
Bio Protoc. 2017 Mar 20;7(6):e2189. doi: 10.21769/BioProtoc.2189.
is one of the most common and potent fungal pathogens of wheat () and other cereals, known for causing devastating yield losses and mycotoxin contaminations of food and feed. The pathogen is mainly considered as a paradigm for the floral disease Fusarium head blight, while its ability to colonize wheat plants via root infection has been examined recently. has a unique infection strategy which comprises complex, specialized structures and processes. Root colonisation negatively affects plant development and leads to systemic plant invasion by tissue-adapted fungal strategies. The pathosystem wheat root - makes available an array of research areas, such as (i) the relatively unknown root interactions with a necrotrophic pathogen; (ii) genes and pathways contributing to (overall) resistance; (iii) induced systemic (whole-plant) resistance; (iv) pathogenic strategies in a variety of host tissues; and (v) age-related changes in the single-genotype responses to seedling and adult plant (root/spike) infection. The presented root rot bioassay allows for efficient infection of wheat roots, evaluation of disease severity and progress as well as statistical analysis of disease dynamics.
是小麦（）和其他谷物中最常见且最具危害性的真菌病原体之一，以造成毁灭性的产量损失以及食物和饲料的霉菌毒素污染而闻名。该病原体主要被视为花部病害镰刀菌穗腐病的典型范例，而其通过根部感染定殖于小麦植株的能力最近已得到研究。具有独特的感染策略，包括复杂的、专门的结构和过程。根部定殖对植物发育产生负面影响，并通过组织适应性真菌策略导致真菌对植物的系统性侵染。小麦根-病原体系统提供了一系列研究领域，例如：（i）与坏死营养型病原体相对未知的根部相互作用；（ii）促成（总体）抗性的基因和途径；（iii）诱导的系统性（全株）抗性；（iv）在多种寄主组织中的致病策略；以及（v）单基因型对幼苗和成株（根/穗）感染反应中与年龄相关的变化。所展示的根腐生物测定法能够有效感染小麦根部，评估病害严重程度和进展情况以及对病害动态进行统计分析。
