Buneeva Olga, Kopylov Arthur, Kaloshina Svetlana, Zgoda Victor, Medvedev Alexei
Institute of Biomedical Chemistry, 10 Pogodinskaya street, Moscow 119121 Russian Federation.
Data Brief. 2021 Aug 11;38:107276. doi: 10.1016/j.dib.2021.107276. eCollection 2021 Oct.
Fractions of 26S and 20S proteasomes isolated from the rabbit brain by the method of salt fractionation (salt-induced precipitation) contain intrinsic proteasome proteins responsible for assembly of the core particle and regulatory particle of proteasome and also proteasome-binding proteins. These proteasome-binding proteins include components of the ubiquitin-proteasome system, some ubiquitinated proteins, as well as cytoskeleton components, protective proteins, regulators of gene expression, cell division, and differentiation, and multifunctional proteins (mainly, glycolytic enzymes: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), aldolase, pyruvate kinase, etc.). The multifunctional proteins also known as "moonlighting proteins" are involved in various (regulatory) processes in the cell and obviously represent important components of the proteasome interactome rather than contaminants of the 26S and 20S proteasome fractions.
通过盐分级分离法（盐诱导沉淀）从兔脑中分离出的26S和20S蛋白酶体组分含有负责蛋白酶体核心颗粒和调节颗粒组装的内在蛋白酶体蛋白以及蛋白酶体结合蛋白。这些蛋白酶体结合蛋白包括泛素-蛋白酶体系统的组分、一些泛素化蛋白，以及细胞骨架组分、保护蛋白、基因表达、细胞分裂和分化的调节因子，还有多功能蛋白（主要是糖酵解酶：甘油醛-3-磷酸脱氢酶（GAPDH）、醛缩酶、丙酮酸激酶等）。这些也被称为“兼职蛋白”的多功能蛋白参与细胞中的各种（调节）过程，显然代表蛋白酶体相互作用组的重要组分，而不是26S和20S蛋白酶体组分的污染物。
