转录组中预测的胰蛋白酶丝氨酸肽酶及其无活性同源物的数据集。
The dataset of predicted trypsin serine peptidases and their inactive homologs in transcriptomes.
作者信息
Zhiganov Nikita I, Tereshchenkova Valeriia F, Oppert Brenda, Filippova Irina Y, Belyaeva Nataliya V, Dunaevsky Yakov E, Belozersky Mikhail A, Elpidina Elena N
机构信息
Division of Entomology, Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
Division of Natural Compounds, Department of Chemistry, Lomonosov Moscow State University, Moscow, Russia.
出版信息
Data Brief. 2021 Aug 16;38:107301. doi: 10.1016/j.dib.2021.107301. eCollection 2021 Oct.
is an important coleopteran model insect and agricultural pest from the Tenebrionidae family. We used RNA-Seq transcriptome data from to annotate trypsin-like sequences from the chymotrypsin S1 family of serine peptidases, including sequences of active serine peptidases (SerP) and their inactive homologs (SerPH) in transcriptomes. A total of 63 S1 family tryspin-like serine peptidase sequences were assembled. Among the sequences, 58 were predicted to be active trypsins and five inactive SerPH. The length of preproenzyme and mature form of the predicted enzyme, position of signal peptide and proenzyme cleavage sites, molecular mass, active site and S1 substrate binding subsite residues, and transmembrane and regulatory domains were analyzed using bioinformatic tools. The data can be used for further physiological, biochemical, and phylogenetic study of tenebrionid pests and other animal systems.
是一种重要的鞘翅目模式昆虫，也是拟步甲科的农业害虫。我们使用来自[具体来源]的RNA测序转录组数据，注释丝氨酸肽酶胰凝乳蛋白酶S1家族中的类胰蛋白酶序列，包括[具体物种]转录组中活性丝氨酸肽酶（SerP）及其无活性同源物（SerPH）的序列。总共组装了63个S1家族类胰蛋白酶样丝氨酸肽酶序列。在这些序列中，58个被预测为活性胰蛋白酶，5个为无活性的SerPH。使用生物信息学工具分析了预测酶的前原酶和成熟形式的长度、信号肽和原酶切割位点的位置、分子量、活性位点和S1底物结合亚位点残基，以及跨膜和调节结构域。这些数据可用于拟步甲科害虫和其他动物系统的进一步生理、生化和系统发育研究。
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