Ali Aysha, Little Haydn A, Carter Jake G, Douglas Craig, Hicks Matthew R, Kenyon David M, Lacomme Christophe, Logan Richard T, Dafforn Timothy R, Tucker James H R
School of Chemistry, University of Birmingham Edgbaston Birmingham B15 2TT UK
School of Biosciences, University of Birmingham Edgbaston Birmingham B15 2TT UK
RSC Chem Biol. 2020 Oct 23;1(5):449-454. doi: 10.1039/d0cb00135j. eCollection 2020 Dec 1.
Nucleic acid detection is an important part of our bio-detection arsenal, with the COVID-19 pandemic clearly demonstrating the importance to healthcare of rapid and efficient detection of specific pathogenic sequences. As part of the drive to establish new DNA detection methodologies and signal read-outs, here we show how linear dichroism (LD) spectroscopy can be used to produce a rapid and modular detection system for detecting quantities of DNA from both bacterial and viral pathogens. The LD sensing method exploits changes in fluid alignment of bionanoparticles (bacteriophage M13) engineered with DNA stands covalently attached to their surfaces, with the read-out signal induced by the formation of complementary duplexes between DNA targets and two M13 bionanoparticles. This new sandwich assay can detect pathogenic material down to picomolar levels in under 1 minute without amplification, as demonstrated by the successful sensing of DNA sequences from a plant virus () and an ampicillin resistance gene, .
核酸检测是我们生物检测手段的重要组成部分，新冠疫情清楚地表明了快速高效检测特定致病序列对医疗保健的重要性。作为建立新的DNA检测方法和信号读出方式的努力的一部分，在此我们展示了如何利用线性二色性（LD）光谱法构建一个快速且模块化的检测系统，用于检测细菌和病毒病原体中的DNA含量。LD传感方法利用了生物纳米颗粒（噬菌体M13）流体排列的变化，这些生物纳米颗粒表面共价连接有DNA链，读出信号由DNA靶标与两个M13生物纳米颗粒之间形成互补双链体诱导产生。这种新的夹心测定法能够在不进行扩增的情况下，在不到1分钟内检测出低至皮摩尔水平的致病物质，对植物病毒（）的DNA序列和氨苄青霉素抗性基因的成功检测证明了这一点。
