Tridgett Matthew, Moore-Kelly Charles, Duprey Jean-Louis H A, Iturbe Lorea Orueta, Tsang Chi W, Little Haydn A, Sandhu Sandeep K, Hicks Matthew R, Dafforn Timothy R, Rodger Alison
School of Biosciences, University of Birmingham, Edgbaston, Birmingham, West Midlands B15 2TT, UK. Email:
School of Chemistry, University of Birmingham, Edgbaston, Birmingham, West Midlands B15 2TT, UK.
RSC Adv. 2018 Aug 23;8(52):29535-29543. doi: 10.1039/c8ra05475d. Epub 2018 Aug 20.
It is a challenge within the field of biomimetics to recreate the properties of light-harvesting antennae found in plants and photosynthetic bacteria. Attempts to recreate these biological structures typically rely on the alignment of fluorescent moieties attachment to an inert linear scaffold, DNA, RNA or amyloid fibrils, to enable Förster resonance energy transfer (FRET) between attached chromophores. While there has been some success in this approach, refinement of the alignment of the chromophores is often limited, which may limit the efficiency of energy transfer achieved. Here we demonstrate how linear dichroism spectroscopy may be used to ascertain the overall alignment of chromophores bound to the M13 bacteriophage, a model linear scaffold, and demonstrate how this may be used to distinguish between lack of FRET efficiency due to chromophore separation, and chromophore misalignment. This approach will allow the refinement of artificial light-harvesting antennae in a directed fashion.
在仿生学领域，重现植物和光合细菌中光捕获天线的特性是一项挑战。重现这些生物结构的尝试通常依赖于将荧光部分附着到惰性线性支架（DNA、RNA或淀粉样纤维）上，以使附着的发色团之间能够发生Förster共振能量转移（FRET）。虽然这种方法取得了一些成功，但发色团排列的优化往往受到限制，这可能会限制所实现的能量转移效率。在这里，我们展示了如何使用线性二色性光谱来确定与M13噬菌体（一种模型线性支架）结合的发色团的整体排列，并展示了如何利用它来区分由于发色团分离导致的FRET效率低下和发色团排列错误。这种方法将使人工光捕获天线能够以定向方式得到优化。
