Department of Nano-Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Clinical Pharmacy, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
J Biomol Struct Dyn. 2022;40(21):10730-10740. doi: 10.1080/07391102.2021.1947892. Epub 2021 Aug 31.
Herein, we report the synthesis and inhibitory potential of indazole (Methyl 1-indazole-4-carboxylate) derivatives () against α-amylase and α-glucosidase enzymes. The described derivatives demonstrated good inhibitory potential with IC values, ranging between 15.04 ± 0.05 to 76.70 ± 0.06 µM ± SEM for α-amylase and 16.99 ± 0.19 to 77.97 ± 0.19 µM ± SEM for α-glucosidase, respectively. In particular, compounds (- and ) displayed significant inhibitory activities against both the screened enzymes, with their inhibitory potential comparable to the standard acarbose (12.98 ± 0.03 and 12.79 ± 0.17 µM ± SEM, respectively). Additionally, the influence of different substituents on enzyme inhibition activities was assessed to study the structure activity relationships. Molecular docking simulations were performed to rationalize the binding of derivatives/compounds with enzymes. All the synthesized derivatives () were characterized with the aid of spectroscopic instruments such as H-NMR, C-NMR, HR-MS, elemental analysis and FTIR.Communicated by Ramaswamy H. Sarma.
在此，我们报告了吲唑（Methyl 1-indazole-4-carboxylate）衍生物（）的合成及其对α-淀粉酶和α-葡萄糖苷酶的抑制潜力。所描述的衍生物表现出良好的抑制潜力，其对α-淀粉酶的 IC 值在 15.04 ± 0.05 至 76.70 ± 0.06 µM ± SEM 之间，对α-葡萄糖苷酶的 IC 值在 16.99 ± 0.19 至 77.97 ± 0.19 µM ± SEM 之间。特别是化合物（-和）对两种筛选出的酶均表现出显著的抑制活性，其抑制潜力可与标准阿卡波糖（12.98 ± 0.03 和 12.79 ± 0.17 µM ± SEM）相媲美。此外，还评估了不同取代基对酶抑制活性的影响，以研究构效关系。进行了分子对接模拟，以合理化衍生物/化合物与酶的结合。所有合成的衍生物（）都借助于光谱仪器（如 H-NMR、C-NMR、HR-MS、元素分析和 FTIR）进行了表征。由 Ramaswamy H. Sarma 传达。
