Effects of oestrogen and anti-oestrogen on the cells of the endosteal surface of male Japanese quail.

1. The endosteal cellular response occurring in the early stages of medullary bone formation was histologically examined in the femurs of male Japanese quail treated with oestrogen and antioestrogen. 2. The numbers of endosteal cells decreased, and preosteoblasts and osteoblasts increased, on the endosteal surface following oestrogen treatment. 3. After oestrogen and anti-oestrogen treatment the numbers of preosteoblasts and osteoblasts on the endosteal surface did not increase. However, endosteal cells slowly decreased in number and osteoclasts increased. 4. This study demonstrates that differentiation of osteoblasts is induced by oestrogen stimulation and suggests that osteoclasts occur by oestrogen inhibition on the endosteal surface.