Tsui F W, Siminovitch L
Mount Sinai Hospital Research Institute, Toronto, Ont., Canada.
Gene. 1987;61(3):349-61. doi: 10.1016/0378-1119(87)90198-3.
The chromosomal gene (HRS) coding for hamster histidyl-tRNA synthetase, like many other housekeeping genes, lacks many of the features associated with promoters of RNA-polymerase-II-transcribed genes. HRS transcripts have multiple start points. Using RNase protection analysis, we also identified a 300-bp exon located only 36 bp away from the 5'-most start point of the HRS transcript. This exon hybridizes to a 3.5-kb transcript which transcribes from a different strand of DNA in the 5' region of the HRS gene. This divergent 3.5-kb transcript also has multiple transcription start points. The identity and function of this 3.5-kb transcript is not known.
编码仓鼠组氨酰 - tRNA合成酶的染色体基因（HRS），与许多其他管家基因一样，缺乏许多与RNA聚合酶II转录基因启动子相关的特征。HRS转录本有多个起始点。利用核糖核酸酶保护分析，我们还鉴定出一个仅距HRS转录本5'端最起始点36 bp的300 bp外显子。该外显子与一个3.5 kb的转录本杂交，该转录本从HRS基因5'区域的不同DNA链转录而来。这个不同的3.5 kb转录本也有多个转录起始点。这个3.5 kb转录本的身份和功能尚不清楚。
