水醇提取物与二甲双胍对戊酸雌二醇诱导的多囊卵巢综合征大鼠形态学卵巢卵泡紊乱的比较作用
The Comparative Effect of Hydro-Alcoholic Extract and Metformin on Morphometric Ovarian Follicles Disorders in Estradilol Valerate Iinduced-Polycystic Ovary Syndrome Rats.
作者信息
Barzegar Bafrouei Mohammad Hossein, Khazali Homayoun, Kalantar Seyyed Mehdi, Khoradmehr Arezoo
机构信息
Department of Physiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Research and Clinical Center for Infertility, Yazd Reproduction Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
Galen Med J. 2018 Aug 11;7:e1045. doi: 10.22086/gmj.v0i0.1045. eCollection 2018.
BACKGROUND
Polycystic ovary syndrome (PCOS) is an endocrine disorder that affects 6-10% of women in reproductive age. The medicinal values of Citrullus colocynthis (CCT) extract stems from its anti-oxidative and anti-inflammatory effects. This study evaluated the comparative effect of CCT and metformin on morphometric ovarian disorders in PCOS rats.
MATERIALS AND METHODS
Fifty female Wistar rats having 2-3 consecutive estrous cycles during two weeks were divided into five groups (n=10 each group). The rats in the control group only received sesame oil as estradiol valerate solvent, whereas the rats in the sham group were injected intramuscularly with 4 mg/rat estradiol valerate-induced PCOS. Following PCOS induction, the rats in the metformin group received 50 mg/kg metformin orally for three weeks. Rats in the Extract group were treated with 50 mg/kg plant extract for 21 days following the induction with PCOS. Additionally, the rats in metformin+ extract group were treated with the combination of 50 mg metformin plus plant extract for three weeks. The ovaries were removed and were fixed for tissue processing. The slices were stained by hematoxylin-eosin after clearing and hydration. Follicular count and morphometric changes were evaluated in primordial, primary, pre-antral and antral follicles.
RESULTS
The mean diameter of primordial follicle was similar in all groups, but mean diameter of primary follicle decreased in the sham group (145.50± 49.26 µm) compared to metformin (278.76± 156.64 µm), extract (311.70± 147.74 µm) and metformin+ extract groups (265.35± 88.16 µm). The diameter of pre-antral and antral follicles in the sham group were significantly larger than those in the control group, but were not significantly different in all other groups including metformin, extract, and metformin+ extract in comparison with control group (P< 0.05).
CONCLUSION
In this study, the data has demonstrated that CCT like metformin could improve follicular morphometric disorders in PCOS rats.
背景
多囊卵巢综合征（PCOS）是一种内分泌紊乱疾病，影响6% - 10%的育龄女性。苦西瓜（CCT）提取物的药用价值源于其抗氧化和抗炎作用。本研究评估了CCT和二甲双胍对PCOS大鼠卵巢形态计量学紊乱的比较效果。
材料与方法
将50只在两周内有2 - 3个连续发情周期的雌性Wistar大鼠分为五组（每组n = 10）。对照组大鼠仅接受芝麻油作为戊酸雌二醇溶剂，而假手术组大鼠肌肉注射4 mg/只戊酸雌二醇诱导PCOS。PCOS诱导后，二甲双胍组大鼠口服50 mg/kg二甲双胍，持续三周。提取物组大鼠在PCOS诱导后用50 mg/kg植物提取物治疗21天。此外，二甲双胍 + 提取物组大鼠用50 mg二甲双胍加植物提取物的组合治疗三周。取出卵巢并固定以进行组织处理。切片在透明和水化后用苏木精 - 伊红染色。评估原始卵泡、初级卵泡、窦前卵泡和窦卵泡中的卵泡计数和形态计量学变化。
结果
所有组中原始卵泡的平均直径相似，但与二甲双胍组（278.76±156.64 µm）、提取物组（311.70±147.74 µm）和二甲双胍 + 提取物组（265.35±88.16 µm）相比，假手术组初级卵泡的平均直径减小（145.50±49.26 µm）。假手术组窦前卵泡和窦卵泡的直径明显大于对照组，但与对照组相比，二甲双胍组、提取物组和二甲双胍 + 提取物组等所有其他组之间无显著差异（P < 0.05）。
结论
在本研究中，数据表明CCT与二甲双胍一样可以改善PCOS大鼠的卵泡形态计量学紊乱。
Zotero 插件