Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, Faculth de Pharmacie, Monastir, Rue Avicenne 5000 Monastir, Tunisie.
Eur Rev Med Pharmacol Sci. 2011 Jun;15(6):665-72.
Inflammations and immune-related diseases including rheumatoid arthritis are widespread in the entire globe. The treatment of these illnesses is mainly based on the use of synthetic and biotechnological drugs, in recent years. Tunisian traditional medicine is a potential source of new remedies namely Citrullus colocynthis Schrad. (Cucurbitaceae): endemic in southern Tunisia and used in folk medicine to treat many inflammation disorders. Our goal was to assess the in vivo analgesic and anti-inflammatory activities of Tunisian Citrullus colocynthis immature fruit and seed organic extracts (petroleum ether, chloroform, ethyl acetate, acetone and finely methanol extract).
Yields of prepared organic extracts are gravimetrically determined. For the analgesic and anti-inflammatory activities, we have used respectively, the acetic acid writhing test in mice and the carrageenan-induced paw edema assay in rats.
All extracts displayed an important analgesic and anti-inflammatory activities at different doses without inducing any side effects. This study has demonstrated the analgesic and anti-inflammatory activities of Citrullus colocynthis immature fruit and seed extracts. Experiment results provide scientific insight into the ancient practice of utilizing Citrullus colocynthis Schrad. as analgesic and as anti-inflammatory agents.
炎症和免疫相关疾病，包括类风湿关节炎，在全球范围内广泛存在。这些疾病的治疗主要基于使用合成药物和生物技术药物。近年来，突尼斯传统医学是一种新疗法的潜在来源，即苦瓜（葫芦科）：在突尼斯南部特有，并在民间医学中用于治疗许多炎症疾病。我们的目标是评估突尼斯苦瓜未成熟果实和种子有机提取物（石油醚、氯仿、乙酸乙酯、丙酮和精细甲醇提取物）的体内镇痛和抗炎活性。
通过称重法确定制备的有机提取物的产率。对于镇痛和抗炎活性，我们分别使用了小鼠醋酸扭体试验和大鼠角叉菜胶诱导的足肿胀试验。
所有提取物在不同剂量下均表现出重要的镇痛和抗炎活性，且无任何副作用。本研究证明了苦瓜未成熟果实和种子提取物的镇痛和抗炎活性。实验结果为苦瓜作为镇痛和抗炎药物的古老应用提供了科学依据。
