Razei Ali, Cheraghali Abdol Majid, Saadati Mojtaba, Fasihi Ramandi Mahdi, Panahi Yunes, Hajizade Abbas, Siadat Seyed Davar, Behrouzi Ava
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Galen Med J. 2019 Oct 29;8:e1296. doi: 10.31661/gmj.v8i0.1296. eCollection 2019.
Final elimination of some intracellular bacterial agents, such as Brucella, is often a complex issue and impossible to achieve, primarily due to the presence and survival of the bacteria within phagocytic cells. By penetrating into the cell membrane, drug delivery nanosystems can reduce the number of intracellular bacteria. The aim of this study was to assess the efficacy of chitosan nanoparticles on the delivery of gentamicin into infected J774A.1 murine cells in vitro.
Chitosan nanoparticles (NPs) were synthesized using ionic gelation technique. The shape, size and charge of NPs, loading rate and release of the drug were investigated. Finally, the effects of gentamicin-loaded chitosan NPs (Gen-Cs) and free gentamicin on J774A.1 murine cells infected with these bacteria were examined.
The mean size and charge of NPs were computed as 100 nm and +28mV, respectively. The loading capacity of NPs was 22%. About 70% of the drug was released from NPs during the first 8 hours. Antimicrobial activity of the two formulations showed that MIC (minimum inhibitory concentration) of the Gen-Cs and free drug was 3.1 and 6.25 µg, respectively. The minimum bactericidal concentration of the NPs-loaded drug and free drug was 6.25 and 12.5 µg, respectively. Cell culture analysis revealed that there was a significant reduction in the load of the intercellular bacteria in J774A.1 murine cells in both formulations.
Our results showed the Gen-Cs have a proper potential for optimal treatment of intracellular bacterial agents.
彻底清除某些细胞内细菌病原体，如布鲁氏菌，通常是一个复杂的问题，且难以实现，这主要是由于这些细菌在吞噬细胞内的存在和存活。通过穿透细胞膜，药物递送纳米系统可以减少细胞内细菌的数量。本研究的目的是评估壳聚糖纳米颗粒在体外将庆大霉素递送至感染的J774A.1小鼠细胞中的效果。
采用离子凝胶化技术合成壳聚糖纳米颗粒（NPs）。研究了纳米颗粒的形状、大小、电荷、药物负载率和释放情况。最后，检测了负载庆大霉素的壳聚糖纳米颗粒（Gen-Cs）和游离庆大霉素对感染这些细菌的J774A.1小鼠细胞的影响。
纳米颗粒的平均大小和电荷分别计算为100 nm和+28 mV。纳米颗粒的负载能力为22%。在最初的8小时内，约70%的药物从纳米颗粒中释放。两种制剂的抗菌活性表明，Gen-Cs和游离药物的最低抑菌浓度（MIC）分别为3.1和6.25 μg。负载纳米颗粒的药物和游离药物的最低杀菌浓度分别为6.25和12.5 μg。细胞培养分析显示，两种制剂中J774A.1小鼠细胞内细菌的负载量均显著降低。
我们的结果表明，Gen-Cs在最佳治疗细胞内细菌病原体方面具有适当的潜力。
